FastQCFastQC Report
Wed 23 Aug 2023
SRR9855612_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9855612_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences77166579
Total Bases11.5 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGA17088232.2144599671834615No Hit
CGTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGAT13813971.7901493339493515No Hit
TCGGCCGAGGTGGGATCCCGAGGCCTGTAGGCACCATCAATAGATCGGAA9365761.2137067784228195No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAG9055461.1734950696725845No Hit
ATTTATCAGACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGA8776551.1373511841181918Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCTGAGCGTCGCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC6468630.8382683389398408No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA6391380.828257528430799No Hit
CTGAGCGTCGCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT6285590.8145482255990641Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCGGTCGGCGTCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT5338320.6917917146489025Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGAGCGTCGCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG5198820.6737139403316039Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAAC4413730.5719742999103278Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CTAAACCATTCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG4161530.5392917573811326Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA4138950.5363656201475512No Hit
TGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG4062670.5264805117251602Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGTCCGGTGCGGAGTGCCCTTCGTCCTGGGACTGTAGGCACCATCAATAG3788400.4909379227502103No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGGCTGTAGGCACCATCAATA3661830.47453574428898815No Hit
CCGCCGAGGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG3626400.46994437838173436Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAA3526870.457046307573127Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GTCTGAGCGTCGCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT3493020.4526596935183559No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA3194180.4139330836475205No Hit
TGAGCGTCGCTTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT3142080.4071814561067946Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCTGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC2840320.36807644407820644No Hit
TTTATCAGACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAA2796070.36234209631089126Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCGGCCGAGGTGGGATCCCGAGGCCTGTAGGCACCATCAATAGATCGGAA2627130.3404491988688523No Hit
GTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATC2499450.323903175751772No Hit
TGGGCCGCCGGTGAAATACCACTACCTGTAGGCACCATCAATAGATCGGA2336990.30285002008447204No Hit
CTGAGCGTCGCTTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC2116210.2742391884445208No Hit
TAGCCGGTCCCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT2113480.27388540834497793Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTGAGCGTCGCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT2028260.26284176728891917Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAG1994620.25848236708795913No Hit
TTTCTGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACG1951240.2528607624292895No Hit
TGCGGTCGGCGTCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC1914030.24803872671354268No Hit
TCGGCCGAGGTGGGATCCCGCTGTAGGCACCATCAATAGATCGGAAGAGC1894520.2455104301047219No Hit
TCTGAGCGTCGCTTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT1851390.2399212228910653No Hit
CCCGCGGGGGCGCGCCGGCCTGTAGGCACCATCAATAGATCGGAAGAGCA1798980.23312942251852323No Hit
GCATTTATCAGACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT1771330.22954626510007653Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TAGTCCGCCCGGAGGATTCAACCCGGCGGCGGGCTGTAGGCACCATCAAT1750340.22682617561677834No Hit
TTCTGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT1679570.21765510688247564No Hit
TTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATC1648330.21360672215364113No Hit
CTGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT1599450.20727237370468374Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CATTTATCAGACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG1465530.189917710360077Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TATCAGACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACT1445340.18730129270082066Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GCGGGGCGGACTGTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT1425020.1846680283701575No Hit
ACCCCCGCGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGA1397650.1811211560901255Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA1352030.17520927032413863No Hit
TCCGGCCGTGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGA1343000.1740390746102662Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCAGGGTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACT1340060.17365808065691238Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCGCCGGCCGAGGTGGGATCCCGAGGCCTGTAGGCACCATCAATAGATCG1298670.16829435966054682No Hit
TTTTATCAGACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGA1281080.1660148754294265Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAAAAGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAAC1276210.1653837732005717Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAGA1272470.16489910742317604No Hit
CCGCGGGGGCGCGCCGGCCTGTAGGCACCATCAATAGATCGGAAGAGCAC1259670.16324035823850633No Hit
TCGCTGAGAAGACGGTCGAACTTGACTATCTCTGTAGGCACCATCAATAG1253080.16238636158796155No Hit
CAGTCCGCCCGGAGGATTCAACCCGGCGGCGGGCTGTAGGCACCATCAAT1165900.15108872456300027No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAAT1164730.15093710452033907No Hit
AGAGCACTGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGA1104010.14306841307556217Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTATCAGACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAAC1075010.13931030945404488Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGATCGGAAGAGCACAC1070390.13871160467020313No Hit
CGGCCGAGGTGGGATCCCGAGGCCTGTAGGCACCATCAATAGATCGGAAG1067750.13836948765086501No Hit
TCGACTGCATAATTTGTGGTAGTGGGGCTGTAGGCACCATCAATAGATCG1010270.13092066709345765No Hit
TCTTCTGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACAC1008150.13064593675974673No Hit
TTCCCGGTCAGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT987860.12801656012248516Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATCGGAAGAGC987180.12792843907204957No Hit
TTCCGGCCGTGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG958610.1242260590559548Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCTTCTGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACAC936860.12140748133981681No Hit
ATCCGAGTCACGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT931760.12074657346154997Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGCGGGGGCGCGCCGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACA928830.12036687540599668No Hit
ATAGCCGGTCCCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC896250.1161448403718921No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAAT878660.11386535614077177No Hit
GCGGCCGAGGTGGGATCCCGAGGCCTGTAGGCACCATCAATAGATCGGAA875950.11351416783682998No Hit
ATCTCGCTGGGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT850680.11023943409490784No Hit
CCGGCCGTGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAA842670.10920141995668876Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TGTCCGGTGCGGAGTGCCCTTCGTCCTGGGCTGTAGGCACCATCAATAGA816240.10577636206990594No Hit
TGCTGAGAAGACGGTCGAACTTGACTATCTCTGTAGGCACCATCAATAGA803150.10408003184902107No Hit
TCTCCCGGGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG802260.10396469694477449Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTGGGGGGGCCGGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACAC798330.10345540910916887No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)