FastQCFastQC Report
Wed 23 Aug 2023
SRR9855613_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9855613_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44529197
Total Bases6.6 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA704840.15828715707583949Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AAGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCA620150.13926817499089417Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCA596990.1340670931029814Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ATCGCTTCTCGGCCTTTTGGCTAAGATCCTGTAGGCACCATCAATAGATC564280.12672135093745346No Hit
TGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCA497400.11170199184144282Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)