FastQCFastQC Report
Thu 24 Aug 2023
SRR9855614_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9855614_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences94628607
Total Bases14.1 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGA20329542.1483503397656483No Hit
CGTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGAT14020201.4816027039265198No Hit
ATTTATCAGACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGA12964151.3700032591624223Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAG8664740.9156575664270319No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA7970340.8422759514995292No Hit
TCTGAGCGTCGCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC7291510.7705397163883011No Hit
CTGAGCGTCGCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT7260940.767309192240355Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCGGTCGGCGTCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT6488820.6857144161490193Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCGGCCGAGGTGGGATCCCGAGGCCTGTAGGCACCATCAATAGATCGGAA6042750.6385753940137785No Hit
CCGCCGAGGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG5600280.5918168065181388Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGAGCGTCGCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG5600060.5917935577346076Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAAC5408420.5715417537531753Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAA4188040.4425765244541749Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA4103650.4336585024441922No Hit
TGAGCGTCGCTTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT3954300.417875748715185Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTCTGAGCGTCGCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT3917860.41402490475211157No Hit
TTTATCAGACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAA3885280.4105819712637216Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA3872780.4092610176539955No Hit
TGTCCGGTGCGGAGTGCCCTTCGTCCTGGGACTGTAGGCACCATCAATAG3516940.37165716705520135No Hit
TGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG3425170.36195925403403645Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTAAACCATTCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG3153980.3333009012803073Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTGAGCGTCGCTTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC2803220.29623388622850594No Hit
TGGGCCGCCGGTGAAATACCACTACCTGTAGGCACCATCAATAGATCGGA2621460.277026163980201No Hit
TAGCCGGTCCCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT2536640.2680627011660438Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCTGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC2519620.2662640907310408No Hit
TCTGAGCGTCGCTTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT2484620.2625654206238078No Hit
TGCGGTCGGCGTCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC2394000.25298903533473766No Hit
GCATTTATCAGACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT2359770.24937173596986376Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGGCTGTAGGCACCATCAATA2337290.24699613299813236No Hit
CCCGCGGGGGCGCGCCGGCCTGTAGGCACCATCAATAGATCGGAAGAGCA2333890.24663683361628688No Hit
TTGAGCGTCGCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT2181590.23054233483538442Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATCTCGCTGGGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT2100390.2219614201866038No Hit
TATCAGACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACT2010620.21247485974299507Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCGGCCGAGGTGGGATCCCGCTGTAGGCACCATCAATAGATCGGAAGAGC1921250.20303056981489753No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAG1876890.19834276964470163No Hit
ACCCCCGCGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGA1860950.19665828960157894Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATCGGAAGAGC1737510.18361360851481204No Hit
CATTTATCAGACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG1719530.18171354884258203Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA1697700.17940663545855642No Hit
TTCTGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT1676530.17716946842512435No Hit
TTTTATCAGACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGA1670660.176549148609997Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCGCGGGGGCGCGCCGGCCTGTAGGCACCATCAATAGATCGGAAGAGCAC1668530.17632405811489965No Hit
CCGGCCGAGGTGGGATCCCGAGGCCTGTAGGCACCATCAATAGATCGGAA1663540.17579673343389698No Hit
CTGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT1650100.17437644411271955Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAAT1633550.1726275015334422No Hit
GCGGGGCGGACTGTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT1610130.17015256285025943No Hit
AGAGCACTGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGA1489550.15741011594939786Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATC1472670.15562630019482376No Hit
TCTCCCGGGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG1470350.1553811312048586Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAAAAGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAAC1467860.15511799724580116Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCAGGGTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACT1439880.15216117468579032Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTTCTGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACG1432120.15134112668487237No Hit
TCGCTGAGAAGACGGTCGAACTTGACTATCTCTGTAGGCACCATCAATAG1424940.15058237093144572No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAAT1361590.14388777803735397No Hit
TTCCCGGTCAGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT1346620.14230580399434603Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTATCAGACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAAC1333950.14096688541552765Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCCGGCCGTGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGA1310360.13847398176325262Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGATCGGAAGAGCACAC1306040.1380174601957313No Hit
ATCCGAGTCACGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT1280140.13528044431637887Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TAGTCCGCCCGGAGGATTCAACCCGGCGGCGGGCTGTAGGCACCATCAAT1224190.12936785595924497No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAGA1190100.1257653512748No Hit
CGCGGGGGCGCGCCGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACA1179170.12461030943845554No Hit
TCTTCTGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACAC1145430.12104479145508293No Hit
CCTTCTGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACAC1141570.12063688098039951No Hit
AAATACCACTACCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT1107150.1169995031206578Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATCCGAGTCACGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT1075330.1136368836117391No Hit
ATAGCCGGTCCCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC1066330.11268579701273632No Hit
CAGTCCGCCCGGAGGATTCAACCCGGCGGCGGGCTGTAGGCACCATCAAT1065360.1125832910126216No Hit
TCGCCCCAGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGA1043600.11028377496881044Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATGTTTAGACCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGA1010090.10674256253185678Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACAGCCCCCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGA1009600.1066907811503555Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATCCGAGTCACGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC983480.10393051648747191No Hit
TCCGCCGAGGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT971970.10271418240363614Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCGACTGCATAATTTGTGGTAGTGGGGCTGTAGGCACCATCAATAGATCG971690.10268459304277827No Hit
CCCGCGGGGGCGCGCCGGCTGTAGGCACCATCAATAGATCGGAAGAGCAC966330.10211816813492774No Hit
AAAAAGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACT955770.10100222652543116Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)