FastQCFastQC Report
Wed 23 Aug 2023
SRR9855615_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9855615_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences64798422
Total Bases9.7 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA24034803.709164399096015Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AAGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCA982800.15167036012080665Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCA963060.1486239896397477Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCA838790.1294460534856852Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA777210.1199427356425439Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATCGCTTCTCGGCCTTTTGGCTAAGATCCTGTAGGCACCATCAATAGATC712080.10989156495199837No Hit
TGAGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCC670980.10354881790176929Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGAAGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC655990.10123548996301177Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)