FastQCFastQC Report
Tue 22 Aug 2023
SRR9855616_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9855616_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24699278
Total Bases3.7 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA9735093.94144719533907Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AAGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCA401710.16264038163382752Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCA391920.1586767030194162Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCA341060.13808500799092185Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA316590.12817783580556485Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATCGCTTCTCGGCCTTTTGGCTAAGATCCTGTAGGCACCATCAATAGATC299560.12128289741910675No Hit
TGAGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCC275950.11172391354921386Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGAAGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC272610.11037164730078346Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)