FastQCFastQC Report
Wed 23 Aug 2023
SRR9855617_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9855617_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24080129
Total Bases3.6 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA8608133.574785666638248Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCA391530.1625946439074309Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AAGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCA391110.16242022623716013Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCA325080.13499927678958865Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA279150.11592545870497621Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATCGCTTCTCGGCCTTTTGGCTAAGATCCTGTAGGCACCATCAATAGATC272300.11308078955889315No Hit
TGAGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCC252320.10478349181601145Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGAAGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC249220.10349612329734613Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)