FastQCFastQC Report
Thu 24 Aug 2023
SRR9855618_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9855618_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences92006483
Total Bases13.8 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGTCAGCCCTCGACACAAGGGTTTGTCTGTAGGCACCATCAATAGATCG12355421.3428858051231019No Hit
AAAAGTCAGCCCTCGACACAAGGGTTTGTCTGTAGGCACCATCAATAGAT9781541.0631359531479971No Hit
TAAAGTCAGCCCTCGACACAAGGGTTTGTCTGTAGGCACCATCAATAGAT8400900.91307696219624No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA6834980.7428802598616883No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT6316210.6864961896217683No Hit
TAGTCAGCCCTCGACACAAGGGTTTGTCTGTAGGCACCATCAATAGATCG4449740.4836333109265789No Hit
AAAAAGTCAGCCCTCGACACAAGGGTTTGTCTGTAGGCACCATCAATAGA4231070.45986650745034996No Hit
AAGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCA3794330.41239811329382087Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAG3717880.40408891621256726No Hit
TGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCA3536720.3843989993618167Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AAAAAGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACT3195830.3473483493549036Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGA2905160.315756010367226No Hit
TGAAAGTCAGCCCTCGACACAAGGGTTTGTCTGTAGGCACCATCAATAGA2654080.28846662903091297No Hit
AGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCA2052100.22303863087560905Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TAGAGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC1716800.18659554675076537Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TAGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCA1685830.1832294796009103Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA1657060.18010252603612725No Hit
ACGTAGCAGAGCAGCTCCCTCGCTGCGATCCTGTAGGCACCATCAATAGA1641820.17844612101953727No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGAT1633460.17753748939626351No Hit
TAGAGCAGTGGCTGGTTGAGATTTAACTGTAGGCACCATCAATAGATCGG1612940.17530721177550065No Hit
TACCTGGTTGATCCTGCCAGTAGCCTGTAGGCACCATCAATAGATCGGAA1508870.16399605232166084No Hit
TTGAAAGTCAGCCCTCGACACAAGGGTTTGTCTGTAGGCACCATCAATAG1476880.16051912341872693No Hit
AAAGTCAGCCCTCGACACAAGGGTTTGTCTGTAGGCACCATCAATAGATC1446230.15718783642670048No Hit
TCGCCTGGGAATACCGGGTGCTGTAGGCTTTCTGTAGGCACCATCAATAG1442590.1567922121313995No Hit
TAGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCC1430680.15549773813221401Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TAAGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCC1291580.140379238276068Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCGCCTGGGAATACCGGGTGCTGTAGGCTTCTGTAGGCACCATCAATAGA1201040.13053862737042127No Hit
TGAGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCC1141660.1240847343333404Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGAAGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC1085960.11803081311128913Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGAGCAGTGGCTGGTTGAGATTTAACTGTAGGCACCATCAATAGATCGGA1033330.11231056402840657No Hit
ACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGATCGGAAGAGC1010110.10978682882596437No Hit
AGTCAGCCCTCGACACAAGGGTTTGTCTGTAGGCACCATCAATAGATCGG994900.10813368444917083No Hit
AAAAGTCAGCCCTCGACACAAGGGTTTGCTGTAGGCACCATCAATAGATC941910.10237430768873103No Hit
TTACCTGGTTGATCCTGCCAGTCTGTAGGCACCATCAATAGATCGGAAGA931760.10127112455760319No Hit
TCGGTCGTCCGACCTGGGTATAGGGGCGACTGTAGGCACCATCAATAGAT929560.10103201097253114No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)