FastQCFastQC Report
Wed 23 Aug 2023
SRR9855619_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9855619_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39218529
Total Bases5.8 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGTCAGCCCTCGACACAAGGGTTTGTCTGTAGGCACCATCAATAGATCG5712671.456625259963218No Hit
AAAAGTCAGCCCTCGACACAAGGGTTTGTCTGTAGGCACCATCAATAGAT4560021.1627208149494848No Hit
TAAAGTCAGCCCTCGACACAAGGGTTTGTCTGTAGGCACCATCAATAGAT3908940.996707449175363No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA2592850.6611288251020327No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT2066410.5268963555466346No Hit
TAGTCAGCCCTCGACACAAGGGTTTGTCTGTAGGCACCATCAATAGATCG2050860.5229313929647897No Hit
AAAAAGTCAGCCCTCGACACAAGGGTTTGTCTGTAGGCACCATCAATAGA1973350.5031677756195292No Hit
AAGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCA1745070.44496059502894664Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCA1620430.4131796988102231Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AAAAAGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACT1467310.3741369290010852Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAG1246610.3178625083057042No Hit
TGAAAGTCAGCCCTCGACACAAGGGTTTGTCTGTAGGCACCATCAATAGA1234260.3147134865767148No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGA947160.24150829318458122No Hit
AGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCA935560.23855050759297985Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TAGAGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC804810.20521167430833523Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TAGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCA778630.1985362582059108Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TAGAGCAGTGGCTGGTTGAGATTTAACTGTAGGCACCATCAATAGATCGG737610.18807691639836874No Hit
TTGAAAGTCAGCCCTCGACACAAGGGTTTGTCTGTAGGCACCATCAATAG685640.17482552698496162No Hit
AAAGTCAGCCCTCGACACAAGGGTTTGTCTGTAGGCACCATCAATAGATC674200.17190853843600307No Hit
TAGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCC671590.17124303667789273Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TACCTGGTTGATCCTGCCAGTAGCCTGTAGGCACCATCAATAGATCGGAA670550.17097785590071468No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA642820.16390721844768832No Hit
TCGCCTGGGAATACCGGGTGCTGTAGGCTTTCTGTAGGCACCATCAATAG618910.1578106103877583No Hit
ACGTAGCAGAGCAGCTCCCTCGCTGCGATCCTGTAGGCACCATCAATAGA612640.15621187627919444No Hit
TAAGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCC606660.15468708681042065Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGAGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCC533300.13598164275870722Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGAT521430.13295501215764619No Hit
TGAAGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC500510.12762079883210306Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCGCCTGGGAATACCGGGTGCTGTAGGCTTCTGTAGGCACCATCAATAGA500030.12749840770417473No Hit
AGAGCAGTGGCTGGTTGAGATTTAACTGTAGGCACCATCAATAGATCGGA471680.12026968171090763No Hit
AGTCAGCCCTCGACACAAGGGTTTGTCTGTAGGCACCATCAATAGATCGG454110.11578965646569764No Hit
TTACCTGGTTGATCCTGCCAGTCTGTAGGCACCATCAATAGATCGGAAGA434410.11076652059030566No Hit
AAAAGTCAGCCCTCGACACAAGGGTTTGCTGTAGGCACCATCAATAGATC432140.11018771254781128No Hit
TTTGATCCTTCGATGTCGGCTCTTCCTATCCTGTAGGCACCATCAATAGA417280.10639868721236331No Hit
ACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGATCGGAAGAGC410020.1045475214024473No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)