FastQCFastQC Report
Wed 23 Aug 2023
SRR9855620_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9855620_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16464408
Total Bases2.4 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA6573813.9927399758315025Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AAGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCA272100.16526558379748607Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA225800.1371443176092332Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATCGCTTCTCGGCCTTTTGGCTAAGATCAAGTGTAGTATCTGTTCTTATC215810.13107668371677864No Hit
TAGAGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC214130.13005630083996947Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ACCTGCGGAAGGATCATTACTGTAGGCACCATCAATAGATCGGAAGAGCA204800.1243895316491185No Hit
AAAAAGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACT188040.11420999771142698Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCA187890.11411889209742615Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC177580.10785689956176986Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)