FastQCFastQC Report
Wed 23 Aug 2023
SRR9855621_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9855621_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46488222
Total Bases6.9 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA18541113.988345693238171Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AAGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCA745840.16043633589600392Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA629500.13541064229128832Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TAGAGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC586400.12613947679048684Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ATCGCTTCTCGGCCTTTTGGCTAAGATCAAGTGTAGTATCTGTTCTTATC554880.1193592648047499No Hit
ACCTGCGGAAGGATCATTACTGTAGGCACCATCAATAGATCGGAAGAGCA554040.11917857387619599No Hit
AAAAAGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACT516310.11106253966864985Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCA515650.11092056822478605Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC489870.10537507758416745Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)