FastQCFastQC Report
Tue 22 Aug 2023
SRR9855622_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9855622_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15850194
Total Bases2.3 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA7588574.787682724892831Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AAGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCA310370.19581463797856352Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA259160.16350588516456013Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TAGAGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC252640.1593923708441676Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ATCGCTTCTCGGCCTTTTGGCTAAGATCAAGTGTAGTATCTGTTCTTATC235180.1483767328021348No Hit
ACCTGCGGAAGGATCATTACTGTAGGCACCATCAATAGATCGGAAGAGCA218140.13762607574393096No Hit
AAAAAGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACT216450.13655984273757152Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCA212850.13428857716189468Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC199550.12589751267397736Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCA181730.11465474807437687Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGAGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCC174280.10995449014693448Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGAAGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC171410.10814378675743654Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCATCAC164820.10398610893973916Illumina Multiplexing PCR Primer 2.01 (96% over 29bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)