FastQCFastQC Report
Tue 29 Aug 2023
SRR9855623_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9855623_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences123580329
Total Bases18.5 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGTCAGCCCTCGACACAAGGGTTTGTCTGTAGGCACCATCAATAGATCG16160211.3076684720591738No Hit
AAAAGTCAGCCCTCGACACAAGGGTTTGTCTGTAGGCACCATCAATAGAT12597881.0194081940014903No Hit
TAAAGTCAGCCCTCGACACAAGGGTTTGTCTGTAGGCACCATCAATAGAT10201220.8254727983447917No Hit
AAGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCA10147060.8210902238332769Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCA9545610.7724214749420193Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AAAAAGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACT8235650.6664207861107085Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA8144310.6590296421690219No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT7208140.5832756765034992No Hit
AGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCA5644460.4567442120986747Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TAGTCAGCCCTCGACACAAGGGTTTGTCTGTAGGCACCATCAATAGATCG5481900.44359001504195705No Hit
AAAAAGTCAGCCCTCGACACAAGGGTTTGTCTGTAGGCACCATCAATAGA4998330.40446000107347185No Hit
TAGAGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC4339680.35116268382810345Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAG4302350.3481419765438559No Hit
TAGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCA4114400.33293324538729785Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TAGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCC3595580.29095083571107827Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGAAAGTCAGCCCTCGACACAAGGGTTTGTCTGTAGGCACCATCAATAGA3433630.2778459992609342No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGA3377570.27330967859779687No Hit
TAAGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCC3225390.26099542104310147Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGAGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCC3108620.2515465062404875Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGAAGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC3033650.24548000677356996Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA2111720.17087832805494474No Hit
TAGAGCAGTGGCTGGTTGAGATTTAACTGTAGGCACCATCAATAGATCGG2088390.168990487151074No Hit
AAAAGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC2081730.16845156643012335Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TAAGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC2078870.1682201380124178Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGAT2063630.16698693203835052No Hit
AAAGTCAGCCCTCGACACAAGGGTTTGTCTGTAGGCACCATCAATAGATC1843320.1491596611625787No Hit
TACCTGGTTGATCCTGCCAGTAGCCTGTAGGCACCATCAATAGATCGGAA1811790.1466082842359159No Hit
TCGCCTGGGAATACCGGGTGCTGTAGGCTTTCTGTAGGCACCATCAATAG1745100.141211794313964No Hit
ACGTAGCAGAGCAGCTCCCTCGCTGCGATCCTGTAGGCACCATCAATAGA1723530.13946637089791208No Hit
GGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAG1685920.13642300628605705Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TTGAAAGTCAGCCCTCGACACAAGGGTTTGTCTGTAGGCACCATCAATAG1663200.13458452598875992No Hit
TTGAAGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACT1541150.1247083587226896Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AAAGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCC1503390.12165285625675912Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAG1496590.12110260687200468Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AGAGCAGTGGCTGGTTGAGATTTAACTGTAGGCACCATCAATAGATCGGA1483140.12001424595657129No Hit
AGAGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCC1441390.11663587657223344Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCGCCTGGGAATACCGGGTGCTGTAGGCTTCTGTAGGCACCATCAATAGA1420130.11491553805460415No Hit
TAGGGACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC1411290.11420021385442339Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCAGGGTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACT1384790.1120558596344245Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGATCGGAAGAGC1359650.11002155529137653No Hit
AGTCAGCCCTCGACACAAGGGTTTGTCTGTAGGCACCATCAATAGATCGG1318930.10672653250502352No Hit
TGGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCC1272550.10297350802488962Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)