FastQCFastQC Report
Wed 23 Aug 2023
SRR9855624_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9855624_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30706734
Total Bases4.6 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGTCAGCCCTCGACACAAGGGTTTGTCTGTAGGCACCATCAATAGATCG4328381.4095865747233163No Hit
AAAAGTCAGCCCTCGACACAAGGGTTTGTCTGTAGGCACCATCAATAGAT3397381.106395750196032No Hit
TAAAGTCAGCCCTCGACACAAGGGTTTGTCTGTAGGCACCATCAATAGAT2766160.9008317198436018No Hit
AAGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCA2727400.8882090814347108Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCA2553320.8315179334930247Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AAAAAGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACT2204410.7178913915104095Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA1670180.5439132667121159No Hit
AGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCA1505840.49039406144593556Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TAGTCAGCCCTCGACACAAGGGTTTGTCTGTAGGCACCATCAATAGATCG1457830.47475905447971123No Hit
AAAAAGTCAGCCCTCGACACAAGGGTTTGTCTGTAGGCACCATCAATAGA1351880.44025522219328184No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT1312920.4275674514912592No Hit
TAGAGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC1165530.3795682080679762Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TAGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCA1104010.3595335147007168Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TAGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCC969360.31568319834991243Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGAAAGTCAGCCCTCGACACAAGGGTTTGTCTGTAGGCACCATCAATAGA935410.3046269915908348No Hit
TAAGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCC873210.2843708484269281Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGAGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCC829090.270002664562112Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAG814440.26523172409022727No Hit
TGAAGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC812140.26448270271921465Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGA619590.20177658750683156No Hit
TAGAGCAGTGGCTGGTTGAGATTTAACTGTAGGCACCATCAATAGATCGG562240.18309990245136457No Hit
TAAGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC558080.1817451507542287Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AAAAGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC557170.18144879882048023Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AAAGTCAGCCCTCGACACAAGGGTTTGTCTGTAGGCACCATCAATAGATC501760.16340389700838912No Hit
TACCTGGTTGATCCTGCCAGTAGCCTGTAGGCACCATCAATAGATCGGAA467520.1522532484242707No Hit
TTGAAAGTCAGCCCTCGACACAAGGGTTTGTCTGTAGGCACCATCAATAG448860.14617640547509872No Hit
GGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAG446160.14529711951782304Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA428340.13949383219980346No Hit
TCGCCTGGGAATACCGGGTGCTGTAGGCTTTCTGTAGGCACCATCAATAG424530.13825306201564777No Hit
TTGAAGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACT414610.13502250027632376Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAG413840.13477174094776737Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AAAGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCC406940.1325246768347295Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGAGCAGTGGCTGGTTGAGATTTAACTGTAGGCACCATCAATAGATCGGA396310.12906289545478852No Hit
AGAGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCC390640.12721639494450956Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TAGGGACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC383210.12479673025467314Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCAGGGTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACT372270.12123399382037829Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGAT367690.11974246430766619No Hit
ACGTAGCAGAGCAGCTCCCTCGCTGCGATCCTGTAGGCACCATCAATAGA362910.11818580250182256No Hit
AGTCAGCCCTCGACACAAGGGTTTGTCTGTAGGCACCATCAATAGATCGG355280.1157010055188546No Hit
TGGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCC338520.11024291935443217Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCGCCTGGGAATACCGGGTGCTGTAGGCTTCTGTAGGCACCATCAATAGA337180.1098065329904509No Hit
TGGAGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC330480.10762460117054455Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TTGAGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACT324480.10567063237659857Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GGGTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCA321000.1045373304761099Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TTACCTGGTTGATCCTGCCAGTCTGTAGGCACCATCAATAGATCGGAAGA314410.10239122141742589No Hit
TGGGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC311740.10152170530411994Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGATCGGAAGAGC309900.10092248820730984No Hit
GAGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCA307180.10003668902072099Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)