FastQCFastQC Report
Thu 24 Aug 2023
SRR9855626_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9855626_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences107467016
Total Bases16.1 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGA22958592.136338278900384No Hit
CGTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGAT17823851.65854144493972No Hit
TCGGCCGAGGTGGGATCCCGAGGCCTGTAGGCACCATCAATAGATCGGAA12883861.1988664503348636No Hit
ATTTATCAGACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGA11521371.0720842942173066Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGGCTGTAGGCACCATCAATA11206991.0428306672253747No Hit
TTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATC10989621.0226039960018989No Hit
TCTGAGCGTCGCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC9425920.8770988858572196No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAG9182890.8544845052736925No Hit
TGTCCGGTGCGGAGTGCCCTTCGTCCTGGGACTGTAGGCACCATCAATAG7177080.6678402608666458No Hit
CTAAACCATTCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG6879070.6401098919504753Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTGAGCGTCGCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT6878140.6400233537702397Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TAGTCCGCCCGGAGGATTCAACCCGGCGGCGGGCTGTAGGCACCATCAAT6402150.5957316242967051No Hit
TCGGCCGAGGTGGGATCCCGCTGTAGGCACCATCAATAGATCGGAAGAGC5801980.5398847214665382No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA5615600.5225417257328518No Hit
GTCTGAGCGTCGCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT5326600.4956497535950938No Hit
GTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATC4646710.43238476073440063No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA4252430.39569629438673537No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA4147500.385932368309175No Hit
CAGTCCGCCCGGAGGATTCAACCCGGCGGCGGGCTGTAGGCACCATCAAT3967620.36919420931907143No Hit
CCGCCGAGGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG3617680.33663166008070794Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG3586740.3337526371812538Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGAGCGTCGCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG3453140.3213209158054598Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCGGTCGGCGTCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT3177470.29566932425108Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCTGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC3144610.2926116418827522No Hit
TGAGCGTCGCTTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT2990780.27829748245731506Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGGGCCGCCGGTGAAATACCACTACCTGTAGGCACCATCAATAGATCGGA2972130.276562066262266No Hit
ACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATCGGAAGAGC2863670.2664696673070368No Hit
TTTATCAGACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAA2850570.2652506886392007Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCATTTATCAGACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT2721340.2532256036587077Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAA2718990.25300693191295087Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAG2587640.24078457710224316No Hit
CCGGCCGAGGTGGGATCCCGAGGCCTGTAGGCACCATCAATAGATCGGAA2426980.22583487383701062No Hit
CTGAGCGTCGCTTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC2300030.21402194697580512No Hit
TCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAAC2244370.2088426834146023Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTCTGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT2208840.20553655272237203No Hit
TAGCCGGTCCCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT2202050.20490473095484477Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCTGAGCGTCGCTTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT2192090.20397793496006253No Hit
TTTCTGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACG2185400.20335541837320578No Hit
TTGAGCGTCGCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT2178340.20269847261786816Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGATCGGAAGAGCACAC2118750.19715351545631452No Hit
CCCGCGGGGGCGCGCCGGCCTGTAGGCACCATCAATAGATCGGAAGAGCA1994530.1855946200274138No Hit
CCGCGGGGGCGCGCCGGCCTGTAGGCACCATCAATAGATCGGAAGAGCAC1905730.1773316195920058No Hit
CTGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT1862310.17329131014487273Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CATTTATCAGACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG1836850.17092221114616227Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATCG1815920.16897463683182568No Hit
TCGACTGCATAATTTGTGGTAGTGGGGCTGTAGGCACCATCAATAGATCG1814460.1688387811940363No Hit
TGCTGAGAAGACGGTCGAACTTGACTATCTCTGTAGGCACCATCAATAGA1753970.16321007740644813No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAGA1739320.16184686843821922No Hit
CCGGCCGAGGTGGGATCCCGCTGTAGGCACCATCAATAGATCGGAAGAGC1646300.15319118937851592No Hit
CGGCCGAGGTGGGATCCCGCTGTAGGCACCATCAATAGATCGGAAGAGCA1595490.14846322707983256No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA1594350.14835714802018882No Hit
CGGCCGAGGTGGGATCCCGAGGCCTGTAGGCACCATCAATAGATCGGAAG1591790.1481189353950239No Hit
TGTCCGGTGCGGAGTGCCCTTCGTCCTGGGCTGTAGGCACCATCAATAGA1566810.14579450126353188No Hit
TCTTCTGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACAC1564910.14561770283079228No Hit
ATCTCGCTGGGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT1553470.14455319016208656No Hit
GTGGGGGGGCCGGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACAC1517620.1412172828917107No Hit
TTTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGAT1513350.14081995167708017No Hit
TTTTATCAGACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGA1496200.13922411319208863Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCTTCTGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACAC1495060.1391180341324449No Hit
TCGCTGAGAAGACGGTCGAACTTGACTATCTCTGTAGGCACCATCAATAG1483990.13808795063222004No Hit
TGGCCGAGGTGGGATCCCGAGGCCTGTAGGCACCATCAATAGATCGGAAG1460860.13593566234313234No Hit
TCCGGCCGTGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGA1377620.12819002995300435Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCGCCGGCCGAGGTGGGATCCCGAGGCCTGTAGGCACCATCAATAGATCG1371430.12761403926950013No Hit
AGCGAGCGCACGGGGTCGGCGGCGACCTGTAGGCACCATCAATAGATCGG1367140.1272148470187355No Hit
TGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATCGGAAGAGCAC1331610.12390871632650524No Hit
TGTGCATTTATCAGACTGTAGGCACCATCAATAGATCGGAAGAGCACACG1328530.12362211676185371No Hit
ATCAAGCACGCAGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT1317710.12261529621330511No Hit
CTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATCGGAAGAGCA1289370.11997820801128414No Hit
ACCCCCGCGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGA1277720.11889415446317035Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGTGGGGGGGCCGGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACA1246010.11594348167255338No Hit
TTCCGGCCGTGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG1234730.11489385729292047Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGGCCGCCGGTGAAATACCACTACCTGTAGGCACCATCAATAGATCGGAA1165640.10846490796766889No Hit
TGTCCGCCCGGAGGATTCAACCCGGCGGCGGGCTGTAGGCACCATCAATA1140110.10608929534248908No Hit
TGCGGTCGGCGTCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC1136700.10577198868162488No Hit
ACAGCCCCCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGA1125070.1046897961696452Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCGGCCGAGGTGGGATCCCGAGGCCTGTAGGCACCATCAATAGATCGGAA1117540.10398911606515622No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAAT1097450.10211970526845186No Hit
TGCGGCCCCGGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT1082670.1007443995653513Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)