FastQCFastQC Report
Wed 23 Aug 2023
SRR9855627_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9855627_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42136089
Total Bases6.3 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCA833180.19773548513247158Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCA773700.18361931977122983Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCA587240.13936746716098877Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGAAGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC522550.1240148320362623Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ATCGCTTCTCGGCCTTTTGGCTAAGATCCTGTAGGCACCATCAATAGATC519660.1233289591732161No Hit
TGAGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCC471270.11184474192657036Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TAGAGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC436540.10360240125750636Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)