FastQCFastQC Report
Sat 23 Sept 2023
SRR9855628_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9855628_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences187347383
Total Bases28.1 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGA28524181.5225288735418312No Hit
CGTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGAT18070620.9645515037698713No Hit
TCTGAGCGTCGCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC15719210.8390408100870029No Hit
ATTTATCAGACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGA15545660.8297772699605844Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTGAGCGTCGCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT14514040.7747127164300982Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAG14359600.7664692065647909No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGGCTGTAGGCACCATCAATA13394040.7149307231048965No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA12877870.6873792306989418No Hit
TCGGCCGAGGTGGGATCCCGAGGCCTGTAGGCACCATCAATAGATCGGAA11621910.6203401303982986No Hit
TGAGCGTCGCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG9981800.5327963401549088Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAAC8731460.4660572173564869Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TGAGCGTCGCTTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT8557620.45677819796393954Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTCTGAGCGTCGCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT8537230.4556898454247424No Hit
TCGGCCGAGGTGGGATCCCGCTGTAGGCACCATCAATAGATCGGAAGAGC7815440.41716301956563756No Hit
ATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAA7763400.4143852919472059Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCGCCGAGGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG7612160.40631258777711354Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGTCCGGTGCGGAGTGCCCTTCGTCCTGGGACTGTAGGCACCATCAATAG7340300.39180157643301583No Hit
TGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG7190440.38380253221898486Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA6785340.36217959874037847No Hit
TAGTCCGCCCGGAGGATTCAACCCGGCGGCGGGCTGTAGGCACCATCAAT6705160.35789984853965107No Hit
CTAAACCATTCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG6624550.3535971463236292Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTGAGCGTCGCTTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC6454380.3445140197127814No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA6345920.33872477418059266No Hit
TCTGAGCGTCGCTTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT5756470.30726183135421753No Hit
TCTGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC5548430.2961573260940613No Hit
TTTATCAGACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAA4689960.2503349619780918Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTGAGCGTCGCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT4512590.24086752255301053Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCTCCCGGGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG4358760.23265657252335356Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATCTCGCTGGGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT4200550.22421183219837132No Hit
GCGGTCGGCGTCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT3945230.2105836727914155Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTCTGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT3728390.19900945186941843No Hit
CAGTCCGCCCGGAGGATTCAACCCGGCGGCGGGCTGTAGGCACCATCAAT3693470.19714553472038623No Hit
CTGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT3579620.19106858834531998Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATCGGAAGAGC3518020.1877805787124339No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAG3492270.1864061266337518No Hit
TTTCTGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACG3426030.18287044874280417No Hit
ACAGCCCCCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGA3163940.16888092853690945Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGATCGGAAGAGCACAC3067470.1637316705939789No Hit
GCATTTATCAGACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT3040440.16228889623721085Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCGGCCGTGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGA2996390.15993764908901878Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATC2922240.15597976087021187No Hit
AAAAAGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAAC2731310.14578853231165764Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TGCTGAGAAGACGGTCGAACTTGACTATCTCTGTAGGCACCATCAATAGA2722370.14531134390065112No Hit
TCTTCTGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACAC2708680.14458061578581005No Hit
CCTTCTGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACAC2670960.14256724365346485No Hit
TCGCTGAGAAGACGGTCGAACTTGACTATCTCTGTAGGCACCATCAATAG2642320.14103853268129185No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAAT2640950.140965406493028No Hit
CGCTGCGATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAA2633130.14054800007534668Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATCCGAGTCACGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT2626090.14017222754587397No Hit
ATGTTTAGACCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGA2545030.1358455057789625Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA2477190.1322244250404074No Hit
TTCCGGCCGTGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG2302540.12290217045625879Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAAT2297860.12265236712700706No Hit
CATTTATCAGACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG2271530.12124695651606728Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGAGCACTGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGA2222230.1186154812741633Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCGCTGCGATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGA2222030.11860480591821237Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCAGGGTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACT2184380.11659517016045001Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GTCTGAGCGTCGCTTCTGTAGGCACCATCAATAGATCGGAAGAGCACACG2179930.11635764349054184No Hit
CCGGCCGAGGTGGGATCCCGCTGTAGGCACCATCAATAGATCGGAAGAGC2179680.11634429929560318No Hit
ATCAAGCACGCAGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT2165810.11560396336040626No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAGA2134740.11394554681342947No Hit
CCGGCCGAGGTGGGATCCCGAGGCCTGTAGGCACCATCAATAGATCGGAA2107240.11247768537017676No Hit
TTTTATCAGACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGA2097030.11193270844888183Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGGCCGAGGTGGGATCCCGCTGTAGGCACCATCAATAGATCGGAAGAGCA2077330.11088118588771534No Hit
TAGCCGGTCCCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT2025210.10809918812690326Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCGACTGCATAATTTGTGGTAGTGGGGCTGTAGGCACCATCAATAGATCG1980820.1057297928735946No Hit
ATCCGAGTCACGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC1961140.1046793378480232No Hit
AAAAAGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACT1951380.10415838047761788Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATCGGAAGAGCAC1944500.10379114823290594No Hit
TGCGGCCCCGGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT1929620.10299690175015683Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TATCAGACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACT1904280.10164433415117413Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)