FastQCFastQC Report
Tue 27 Jun 2023
SRR987020.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR987020.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8433879
Total Bases430.1 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC33

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGACGTGGGGGTTCGAGTCCCTTCACCCGCACCAAAAAAAAAAAAAAAA1508981.7891885809601966No Hit
ACGGCGGTAACACGGGTTCGAATCCCGTACGGGAAAAAAAAAAAAAAAAA734550.8709515514747129No Hit
TAACCCGTGGAGGTTCAAGTCCTCTCGGCCGCACCAAAAAAAAAAAAAAA462190.5480159248194099No Hit
ACCCGTGGAGGTTCAAGTCCTCTCGGCCGCACCAAAAAAAAAAAAAAAAA434270.5149113474357411No Hit
GCCGGTGTAGCTCAATTGGTAGAGCAACTGAAAAAAAAAAAAAAAAAAAA402110.47677942735483875No Hit
TCCACAGTAGCTCAGTGGTAGAGCTATCGGCAAAAAAAAAAAAAAAAAAA335670.39800191584441746No Hit
ACGACGTGGGGGTTCGAGTCCCTTCACCCGCACAAAAAAAAAAAAAAAAA288650.3422505824425511No Hit
ATCAAAAGGTTAGGGGTTCGACTCCTCTCGGAAAAAAAAAAAAAAAAAAA274750.32576943539265857No Hit
ATGACGTGGGGGTTCAAGTCCCTTCACCCGCACCAAAAAAAAAAAAAAAA271240.3216076493390526No Hit
ACCGATCGGTCGCAGGTTCGAATCCTGCCTGTGGAAAAAAAAAAAAAAAA250450.29695707040615593No Hit
ACGTGGGGGTTCGAGTCCCTTCACCCGCACCAAAAAAAAAAAAAAAAAAA238390.2826576003758176No Hit
ACGCAGGAGGTCGCGGGTTCGAGTCCCGTCCGGAAAAAAAAAAAAAAAAA226900.269033975943928No Hit
ACCCGAAGGTCGCAGGTTCAAATCCTGCCCCCGCAAAAAAAAAAAAAAAA222230.26349678481277716No Hit
ACGCAGGAGGTCGCGGGTTCGAGTCCCGTCCGAAAAAAAAAAAAAAAAAA198250.2350638419166317No Hit
TACCCGAAGGTCGCAGGTTCAAATCCTGCCCCCGCAAAAAAAAAAAAAAA198090.2348741308714531No Hit
TCGGTCGCAGGTTCGAATCCTGCCTGTGGAGCCAAAAAAAAAAAAAAAAA197130.23373586460038137No Hit
GCGGAAGTAGTTCAGTGGTAGAACACCACCAAAAAAAAAAAAAAAAAAAA193510.22944365220321514No Hit
TCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCAAAAAAAAAAAAAAAAA175910.20857543723356714No Hit
TTCTGGACGTGGGTTCGACTCCCACCGTCTCCACCAAAAAAAAAAAAAAA170770.20248096990720402No Hit
ACCATGGGGTCGCAGGTTCGAATCCTGTCTTCCCGAAAAAAAAAAAAAAA166620.1975603396728836No Hit
TCTGGACGTGGGTTCGACTCCCACCGTCTCCACCAAAAAAAAAAAAAAAA159580.18921305368502442No Hit
AACCCGTGGAGGTTCAAGTCCTCTCGGCCGCACCAAAAAAAAAAAAAAAA154620.18333201128448723No Hit
ACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAAAAAAAAAAAAAAAA152970.18137561613108275No Hit
ACTACCGTGCCGGTTCAAGTCCGGCCCTCGGCACCAAAAAAAAAAAAAAA150510.1784588088114615No Hit
GCGGAAGTAGTTCAGTGGTAGAACACCAAAAAAAAAAAAAAAAAAAAAAA148800.176431272016115No Hit
ACGACGTGGGGGTTCGAGTCCCTTCACCCGCACCGAAAAAAAAAAAAAAA146740.17398874230944028No Hit
ACGGCGGTAACACGGGTTCGAATCCCGTACGGCAAAAAAAAAAAAAAAAA143560.17021823528651525No Hit
AGGCTTGCCTCTTGGGGTTGTAGGACACTCAAAAAAAAAAAAAAAAAAAA142870.16940010640418246No Hit
TCCACAGTAGCTCAGTGGTAGAGCTATCGGCTAAAAAAAAAAAAAAAAAA139260.16511975094733988No Hit
ACGGCGGTAACACGGGTTCGAATCCCGTACGAAAAAAAAAAAAAAAAAAA137670.16323449743587737No Hit
ACGTGGGGGTTCAAGTCCCTTCACCCGCACCAAAAAAAAAAAAAAAAAAA131040.15537334600128838No Hit
ACGGCGGTAACACGGGTTCGAATCCCGTACGGGTAAAAAAAAAAAAAAAA121390.14393139858895296No Hit
TCGGTCGTAGGTTCGAATCCTACCTGCGGAGCAAAAAAAAAAAAAAAAAA119060.14116873149353934No Hit
TAACCCGTGGAGGTTCAAGTCCTCTCGGCCGCACAAAAAAAAAAAAAAAA118130.14006603604343862No Hit
ACGACGTGGGGGTTCGAGTCCCTTCACCCGCATCAAAAAAAAAAAAAAAA113780.1349082670026449No Hit
TCGGTCGCAGGTTCGAATCCTGCCTGTGGAGCAAAAAAAAAAAAAAAAAA110110.1305567699038604No Hit
TTGTAACACCCGAAGTCGGTGAGGTAACCTAAAAAAAAAAAAAAAAAAAA108670.12884937049725279No Hit
GCGGGTGTAGTTTAGTGGTAAAACCTCAAAAAAAAAAAAAAAAAAAAAAA106690.1265016963131674No Hit
TTGACGTGGGGGTTCAAGTCCCTTCACCCGCACCAAAAAAAAAAAAAAAA103680.12293275727574464No Hit
TAGCCCGCGGGGGTTCGAATCCCTCTTCCTCCGCCAAAAAAAAAAAAAAA103560.12279047399186067No Hit
ACGACGTGGGGGTTCGAGTCCCTTCACCCGCACTAAAAAAAAAAAAAAAA100610.1192926765963799No Hit
GCGGAAGTAGTTCAGTGGTAGAACACCAAAAAAAAAAAAAAAAAAAAAAT98570.11687386077035251No Hit
GCGGAAGTAGTTCAGTGGTAGAACACCACCTAAAAAAAAAAAAAAAAAAA94950.1125816483731863No Hit
GTGTGTCGGCGGTTCGATTCCGTCCCGAGCCACCAAAAAAAAAAAAAAAA94590.11215479852153441No Hit
ACCCGTGGAGGTTCAAGTCCTCTCGGCCGCACAAAAAAAAAAAAAAAAAA93160.11045925605525049No Hit
TGCGTTGGTTCGAATCCAGCTAGCCCAGCCAAAAAAAAAAAAAAAAAAAA92270.10940398836644444No Hit
TTGTAACACCCGAAGTCGGTGAGGTAACCTTAAAAAAAAAAAAAAAAAAA91300.10825386515504905No Hit
TCGGTCGGGAGTTCGAATCTCTCCTGGGACGCCAAAAAAAAAAAAAAAAA91160.10808786799051777No Hit
ACGACGTGGGGGTTCGAGTCCCTTCACCCGCACCTAAAAAAAAAAAAAAA90190.10693774477912238No Hit
TGAGCCATTAGCTCAGTTGGTAGAGCATCTGACAAAAAAAAAAAAAAAAA89250.10582319238869801No Hit
TTGCGTTGGTTCGAATCCAGCTAGCCCAGCCAAAAAAAAAAAAAAAAAAA89200.1057639076870797No Hit
TGGAGTGTCGGTTCGACCCCGACCACCGGTACCAAAAAAAAAAAAAAAAA86980.10313166693522638No Hit
GCGGAAGTAGTTCAGTGGTAGAACACCACAAAAAAAAAAAAAAAAAAAAA86300.10232539499321724No Hit
ACGGCGGTAACACGGGTTCGAATCCCGTACGGAAAAAAAAAAAAAAAAAA85700.10161397857379742No Hit
GCGGGTGTAGTTTAGTGGTAAAACCTCAAAAAAAAAAAAAAAAAAAAAAT85280.10111598708020354No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)