FastQCFastQC Report
Tue 27 Jun 2023
SRR987022.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR987022.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3692648
Total Bases369.2 Mbp
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGCTTGCCTCTTGGGGTTGTAGGACACTCAAAAAAAAAAAAAAAAAAAA553631.499276400025131No Hit
ACGACGTGGGGGTTCGAGTCCCTTCACCCGCACCAAAAAAAAAAAAAAAA317240.8591124851326203No Hit
GAGGCTTGCCTCTTGGGGTTGTAGGACACTCAAAAAAAAAAAAAAAAAAA290370.7863462750849797No Hit
GCCGGTGTAGCTCAATTGGTAGAGCAACTGAAAAAAAAAAAAAAAAAAAA196900.5332216880677498No Hit
CGGGAAACCGGGGCTAATACCGGATGGTTGTTTGAAAAAAAAAAAAAAAA183790.4977187102588711No Hit
AGGCTTGCCTCTTGGGGTTGTAGGACACCCAAAAAAAAAAAAAAAAAAAA160340.43421414659615537No Hit
GAGGCTTGCCTCTTGGGGTTGTAGGACACTAAAAAAAAAAAAAAAAAAAA143940.3898015732883286No Hit
ACTACCGTGCCGGTTCAAGTCCGGCCCTCGGCACCAAAAAAAAAAAAAAA141150.3822460196585215No Hit
ACCCGTGGAGGTTCAAGTCCTCTCGGCCGCACCAAAAAAAAAAAAAAAAA139820.37864426828660624No Hit
AGGCTTGCCTCTTGGGGTTGTAGGACACTCTAAAAAAAAAAAAAAAAAAA139730.37840054075016083No Hit
AGGCTTGCCTCTTGGGGTTGTAGGACACTCAAAAAAAAAAAAAAAAAAAG131320.3556255565112082No Hit
AGGCTTGCCTCTTGGGGTTGTAGGACACTAAAAAAAAAAAAAAAAAAAAA126300.342030976145032No Hit
AACCCGTGGAGGTTCAAGTCCTCTCGGCCGCACCAAAAAAAAAAAAAAAA112790.3054447648408405No Hit
GAGGCTTGCCTCTTGGGGTTGTAGGACACAAAAAAAAAAAAAAAAAAAAA104620.2833197206990756No Hit
TAACCCGTGGAGGTTCAAGTCCTCTCGGCCGCACCAAAAAAAAAAAAAAA103950.2815053045944265No Hit
ATGACGTGGGGGTTCAAGTCCCTTCACCCGCACCAAAAAAAAAAAAAAAA95150.25767416769754387No Hit
GCCGGTGTAGCTCAATTGGTAGAGCAACTGACAAAAAAAAAAAAAAAAAA79740.2159425972906164No Hit
AGGCTTGCCTCTTGGGGTTGTAGGACACTAAAAAAAAAAAAAAAAAAAAG79370.214940606307452No Hit
TGACGTGGGGGTTCAAGTCCCTTCACCCGCACCAAAAAAAAAAAAAAAAA78230.21185339084581037No Hit
AGGCTTGCCTCTTGGGGTTGTAGGACACAAAAAAAAAAAAAAAAAAAAAA76200.20635598085709767No Hit
ACGACGTGGGGGTTCGAGTCCCTTCACCCGCACAAAAAAAAAAAAAAAAA69090.1871015054779118No Hit
TGGAGTGTCGGTTCGACCCCGACCACCGGTACCAAAAAAAAAAAAAAAAA68460.18539541272279406No Hit
AGGCTTGCCTCTTGGGGTTGTAGGACACAAAAAAAAAAAAAAAAAAAAGA65460.1772711615079477No Hit
AAGCGGCGCGAGGGTTCAAATCCCTCCTTCTCCGCCAAAAAAAAAAAAAA63800.17277574250239935No Hit
GGGGCCTTAGCTCAGCTGGGAGAGCGCCTGCAAAAAAAAAAAAAAAAAAA63510.17199039821829754No Hit
AGGCTTGCCTCTTGGGGTTGTAGGACACTCTGAAAAAAAAAAAAAAAAAA63190.17112381142204727No Hit
GAGGCTTGCCTCTTGGGGTTGTAGGACACTCTAAAAAAAAAAAAAAAAAA60340.16340577276794324No Hit
TAGGCTTGCCTCTTGGGGTTGTAGGACACTCAAAAAAAAAAAAAAAAAAA58460.1583145753399728No Hit
AGGCTTGCCTCTTGGGGTTGTAGGACACTAAAAAAAAAAAAAAAAAAAGA54890.14864671639430566No Hit
GCCGGTGTAGCTCAATTGGTAGAGCAACTGAAAAAAAAAAAAAAAAAAAG54830.14848423137000874No Hit
GCGGAAGTAGTTCAGTGGTAGAACACCACCAAAAAAAAAAAAAAAAAAAA52170.14128072862617827No Hit
ACCGTGCCGGTTCAAGTCCGGCCCTCGGCACCAAAAAAAAAAAAAAAAAA51080.13832891735145075No Hit
ATCAGAGGGTCGAAGGTTCGAGTCCTTCATGAAAAAAAAAAAAAAAAAAA49880.13507921686551225No Hit
AGGCTTGCCTCTTGGGGTTGTAGGACACTCAAAAAAAAAAAAAAAAAAGA49280.13345436662254295No Hit
GCGGAAGTAGTTCAGTGGTAGAACACCAAAAAAAAAAAAAAAAAAAAGAT49130.13304815406180065No Hit
AGGCTTGCCTCTTGGGGTTGTAGGACACTCGAAAAAAAAAAAAAAAAAAA48670.13180243554219087No Hit
AGCGAAGGTGCGAATTGAAGCCCCAGTAAACGGCGGCCGTAAAAAAAAAA48610.13163995051789393No Hit
TCGGGAAACCGGGGCTAATACCGGATGGTTGTTTGAAAAAAAAAAAAAAA46560.1260883788544156No Hit
ATAACCCGTGGAGGTTCAAGTCCTCTCGGCCGCACCAAAAAAAAAAAAAA45530.123299052603985No Hit
GAGGCTTGCCTCTTGGGGTTGTAGGACACAAAAAAAAAAAAAAAAAAAAG42240.11438945710503681No Hit
TACCGTGCCGGTTCAAGTCCGGCCCTCGGCACCAAAAAAAAAAAAAAAAA41970.11365827449570064No Hit
GCGCCCGTAGCTCAATTGGATAGAGCGTTTGACAAAAAAAAAAAAAAAAA41890.11344162779663806No Hit
TGCGTTGGTTCGAATCCAGCTAGCCCAGCCAAAAAAAAAAAAAAAAAAAA41200.1115730500172234No Hit
TAAGCGGCGCGAGGGTTCAAATCCCTCCTTCTCCGCCAAAAAAAAAAAAA41050.1111668374564811No Hit
ATCAGTAGGTTGGGGGTTCAAGTCCTCTTGCCGGCACCAAAAAAAAAAAA41010.11105851410694982No Hit
GCGGGTGTAGTTTAGTGGTAAAACCTCAAAAAAAAAAAAAAAAAAAAGAT40980.11097727159480135No Hit
AGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCAAAAAAAAAAAA40750.11035441233499645No Hit
CGGGAAACCGGGGCTAATACCGGATGGTTGTTTAAAAAAAAAAAAAAAAA39840.10789005613315973No Hit
TTGACGTGGGGGTTCAAGTCCCTTCACCCGCACCAAAAAAAAAAAAAAAA37860.10252805033136111No Hit
AGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCAAAAAAAAAAAAAAA37860.10252805033136111No Hit
AGGCTTGCCTCTTGGGGTTGTAGGACACCCAAAAAAAAAAAAAAAAAAAG37300.10101152343792313No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)