FastQCFastQC Report
Tue 27 Jun 2023
SRR987023.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR987023.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6567480
Total Bases656.7 Mbp
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGCTTGCCTCTTGGGGTTGTAGGACACTCAAAAAAAAAAAAAAAAAAAA988361.5049303538038943No Hit
AGGCTTGCCTCTTGGGGTTGTAGGACACAAAAAAAAAAAAAAAAAAAAGA668871.0184576123566422No Hit
GAGGCTTGCCTCTTGGGGTTGTAGGACACAAAAAAAAAAAAAAAAAAAAA619790.9437257517343028No Hit
CGGGAAACCGGGGCTAATACCGGATGGTTGTTTGAAAAAAAAAAAAAAAA572430.8716128560726488No Hit
AGGCTTGCCTCTTGGGGTTGTAGGACACAAAAAAAAAAAAAAAAAAAAAA538860.8204973597178826No Hit
AGGCTTGCCTCTTGGGGTTGTAGGACACTAAAAAAAAAAAAAAAAAAAAA503740.7670217495904061No Hit
AGGCTTGCCTCTTGGGGTTGTAGGACACTAAAAAAAAAAAAAAAAAAAAG434480.6615627303014247No Hit
GAGGCTTGCCTCTTGGGGTTGTAGGACACAAAAAAAAAAAAAAAAAAAAG365960.5572304750071565No Hit
GAGGCTTGCCTCTTGGGGTTGTAGGACACTAAAAAAAAAAAAAAAAAAAA351910.535837185648072No Hit
AGGCTTGCCTCTTGGGGTTGTAGGACACTAAAAAAAAAAAAAAAAAAAGA300650.4577859392034692No Hit
AGGCTTGCCTCTTGGGGTTGTAGGACACAAAAAAAAAAAAAAAAAAAAAG296650.4516953230158295No Hit
ACGACGTGGGGGTTCGAGTCCCTTCACCCGCACCAAAAAAAAAAAAAAAA293800.44735575898213625No Hit
AGGCTTGCCTCTTGGGGTTGTAGGACACAAAAAAAAAAAAAAAAAAAGAT290310.44204169635842055No Hit
AGGCTTGCCTCTTGGGGTTGTAGGACACTCAAAAAAAAAAAAAAAAAAAG276780.4214401871037293No Hit
AGGCTTGCCTCTTGGGGTTGTAGGACACCCAAAAAAAAAAAAAAAAAAAA238110.3625591551097224No Hit
GAGGCTTGCCTCTTGGGGTTGTAGGACACTCAAAAAAAAAAAAAAAAAAA235110.3579911929689927No Hit
GCCGGTGTAGCTCAATTGGTAGAGCAACTGAAAAAAAAAAAAAAAAAAAA189090.28791865373019787No Hit
AGGCTTGCCTCTTGGGGTTGTAGGACAAAAAAAAAAAAAAAAAAAAGATC164560.25056794995949744No Hit
ACTACCGTGCCGGTTCAAGTCCGGCCCTCGGCACCAAAAAAAAAAAAAAA163950.24963913099088236No Hit
GAGGCTTGCCTCTTGGGGTTGTAGGACACAAAAAAAAAAAAAAAAAAAGA160250.24400531101731565No Hit
GCGGAAGTAGTTCAGTGGTAGAACACCACCAAAAAAAAAAAAAAAAAAAA145680.221820241553838No Hit
AGGCTTGCCTCTTGGGGTTGTAGGACACTCTAAAAAAAAAAAAAAAAAAA145200.22108936761132125No Hit
TCGGGAAACCGGGGCTAATACCGGATGGTTGTTTGAAAAAAAAAAAAAAA142600.21713046708935543No Hit
TAGGCTTGCCTCTTGGGGTTGTAGGACACTCAAAAAAAAAAAAAAAAAAA136230.20743116081053922No Hit
AGGCTTGCCTCTTGGGGTTGTAGGACACAAAAAAAAAAAAAAAAAAAAAT131480.20019855408771708No Hit
TAGGCTTGCCTCTTGGGGTTGTAGGACACAAAAAAAAAAAAAAAAAAAAA128640.19587421659449286No Hit
TATTGGATATCCGCGTCCAAGCAGTTAGGCTGGGAAAAAAAAAAAAAAAA128100.1950519834091615No Hit
AAGGTGCGAATTGAAGCCCCAGTAAACGGCGGCCGTAAAAAAAAAAAAAA124540.18963133500216217No Hit
GCGGAAGTAGTTCAGTGGTAGAACACCAAAAAAAAAAAAAAAAAAAAGAT123590.18818481365759773No Hit
TGAGGCTTGCCTCTTGGGGTTGTAGGACACAAAAAAAAAAAAAAAAAAAA121390.1848349747543959No Hit
CGGGAAACCGGGGCTAATACCGGATGGTTGTTTAAAAAAAAAAAAAAAAA120070.1828250714124748No Hit
ACCGTGCCGGTTCAAGTCCGGCCCTCGGCACCAAAAAAAAAAAAAAAAAA119810.18242918136027822No Hit
AGGCTTGCCTCTTGGGGTTGTAGGACACTAAAAAAAAAAAAAAAAAAGAT119600.18210942401042715No Hit
GGGGCCTTAGCTCAGCTGGGAGAGCGCCTGCAAAAAAAAAAAAAAAAAAA118880.181013113096652No Hit
TGCGTTGGTTCGAATCCAGCTAGCCCAGCCAAAAAAAAAAAAAAAAAAAA117440.1788204912691017No Hit
GCGGGTGTAGTTTAGTGGTAAAACCTCAAAAAAAAAAAAAAAAAAAAGAT117070.178257109271745No Hit
ACCCGTGGAGGTTCAAGTCCTCTCGGCCGCACCAAAAAAAAAAAAAAAAA116550.17746532916735186No Hit
TAGGCTTGCCTCTTGGGGTTGTAGGACACTAAAAAAAAAAAAAAAAAAAA111290.16945616888060566No Hit
GGGAAACCGGGGCTAATACCGGATGGTTGTTTGAAAAAAAAAAAAAAAAA105150.1601070730325787No Hit
TACCGTGCCGGTTCAAGTCCGGCCCTCGGCACCAAAAAAAAAAAAAAAAA104140.15856919244519968No Hit
AGGCTTGCCTCTTGGGGTTGTAGGACAAAAAAAAAAAAAAAAAAAGATCG103920.1582342085548795No Hit
GAGGCTTGCCTCTTGGGGTTGTAGGACAAAAAAAAAAAAAAAAAAAAGAT103060.15692472607453697No Hit
AGGCTTGCCTCTTGGGGTTGTAGGACACTCAAAAAAAAAAAAAAAAAAGA102670.1563308909962421No Hit
TTGTAACACCCGAAGTCGGTGAGGTAACCTAAAAAAAAAAAAAAAAAAAA101130.1539860037640008No Hit
GAGGCTTGCCTCTTGGGGTTGTAGGACACTAAAAAAAAAAAAAAAAAAAG100370.15282878668834926No Hit
AGGCTTGCCTCTTGGGGTTGTAGGACACAAAAAAAAAAAAAAAAAAGATC95860.1459616169367855No Hit
TAGGCTTGCCTCTTGGGGTTGTAGGACACAAAAAAAAAAAAAAAAAAAAG93050.14168295906496858No Hit
AACCCGTGGAGGTTCAAGTCCTCTCGGCCGCACCAAAAAAAAAAAAAAAA88590.13489192201575034No Hit
AGGCTTGCCTCTTGGGGTTGTAGGACACCAAAAAAAAAAAAAAAAAAAAA87210.13279065943101465No Hit
AGCGAAGGTGCGAATTGAAGCCCCAGTAAACGGCGGCCGAAAAAAAAAAA82610.125786450815229No Hit
ACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAAAAAAAAAAAAAA81790.12453787449676283No Hit
TTCTGGACGTGGGTTCGACTCCCACCGTCTCCACCAAAAAAAAAAAAAAA79060.12038102894869873No Hit
TCTGGACGTGGGTTCGACTCCCACCGTCTCCACCAAAAAAAAAAAAAAAA78520.11955879576336739No Hit
TAACCCGTGGAGGTTCAAGTCCTCTCGGCCGCACCAAAAAAAAAAAAAAA77710.11832544598537034No Hit
TCCCCCTGTGAGAGTAGGACGCCGCCAAGCAAAAAAAAAAAAAAAAAAAA74080.11279821179508731No Hit
AGAGGCTTGCCTCTTGGGGTTGTAGGACACAAAAAAAAAAAAAAAAAAAA73600.11206733785257056No Hit
AGGCTTGCCTCTTGGGGTTGTAGGACACCAAAAAAAAAAAAAAAAAAAAG73510.11193029898834865No Hit
TTCTGGACGTGGGTTCGACTCCCACCGTCTCCAAAAAAAAAAAAAAAAAA71910.10949405251329278No Hit
TTGCGTTGGTTCGAATCCAGCTAGCCCAGCCAAAAAAAAAAAAAAAAAAA71760.10926565440625628No Hit
AGGTGCGAATTGAAGCCCCAGTAAACGGCGGCCGTAAAAAAAAAAAAAAA71210.10842819468045582No Hit
AGGCTTGCCTCTTGGGGTTGTAGGACACTCGAAAAAAAAAAAAAAAAAAA71090.10824547619482662No Hit
AAGGTGCGAATTGAAGCCCCAGTAAACGGCGGCCGAAAAAAAAAAAAAAA69120.10524584772241406No Hit
GCGGAAGTAGTTCAGTGGTAGAACACCAAAAAAAAAAAAAAAAAAAGATC68940.10497176999397029No Hit
TCTGGACGTGGGTTCGACTCCCACCGTCTCCAAAAAAAAAAAAAAAAAAA68790.10474337188693379No Hit
GAGGCTTGCCTCTTGGGGTTGTAAAAAAAAAAAAAAAAAAAAGATCGGAA68730.10465201264411922No Hit
AGGCTTGCCTCTTGGGGTTGTAGGACACCCAAAAAAAAAAAAAAAAAAAG67530.1028248277878273No Hit
AGCGAAGGTGCGAATTGAAGCCCCAGTAAACGGCGGCCGTAAAAAAAAAA67480.1027486950854818No Hit
ATGACGTGGGGGTTCAAGTCCCTTCACCCGCACCAAAAAAAAAAAAAAAA65850.10026676898901862No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)