FastQCFastQC Report
Wed 28 Jun 2023
SRR989509.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR989509.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences72957922
Total Bases3.6 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA21955633.0093551732462993No Hit
TCGCCGCGACCGGCTCCGGGACGGCCGGGCTGTAGGCACCATCAATAGAT11588281.5883511594532531No Hit
TGGCCGCCGGTGAAATACCACTACTCTCACTGTAGGCACCATCAATAGAT8682331.190046229660982No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA8321251.140554688495651No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA8067971.1058387874588862No Hit
CGTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGAT7913431.084656714866413No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT7469441.0238010890715883No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA6488560.8893564704323679No Hit
TGCGCCGCGACCGGCTCCGGGACGGCCGGGCTGTAGGCACCATCAATAGA5678100.7782705214657841No Hit
TTGCGCCGCGACCGGCTCCGGGACGGCCGGGCTGTAGGCACCATCAATAG4971560.6814283992353839No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAG4836410.6629040229517501No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGCTGTAGGCACCATCAATAGATC4746840.6506270833755381No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCTGTAGGCACCATCAATAGATCGGA4583240.6282031990987902No Hit
GCGCCGCGACCGGCTCCGGGACGGCCGGGCTGTAGGCACCATCAATAGAT4401510.6032943208004197No Hit
TTTTCGCCGAATCCCGGGGCCGAGGAAGCTGTAGGCACCATCAATAGATC4142460.5677875529404469No Hit
TGGCCGCCGGTGAAATACCACTACTCTCATCTGTAGGCACCATCAATAGA3795250.5201971075875763No Hit
TTGACGTACGCAGTTTTATCCGGTAAAGCCTGTAGGCACCATCAATAGAT3655750.501076497217122No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCTGTAGGCACCATCAATAGAT3647450.49993885516640674No Hit
TCGTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGA3059390.4193362305466979No Hit
TGGGCCGCCGGTGAAATACCACTACTCTCACTGTAGGCACCATCAATAGA2804860.3844489978757893No Hit
TTTTCGCCGAATCCCGGGGCCGAGGAAGCCTGTAGGCACCATCAATAGAT2551790.3497618805535607No Hit
GCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA2531110.3469273699982847No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAAT2529340.34668476440433704No Hit
TCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCTGTAGGCACCATCAA2504320.34325539041531367No Hit
ACGTACGCAGTTTTATCCGGTAAAGCCTGTAGGCACCATCAATAGATCGG2287610.31355196766706156No Hit
TTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATCG2221220.3044522019144131No Hit
CCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT2210220.30294448353394715No Hit
TGTGCGCCGCGACCGGCTCCGGGACGGCCGGGCTGTAGGCACCATCAATA2186910.2997494912204325No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAG2121300.2907566364074898No Hit
TCTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCG2050830.28109764420099576No Hit
TTACCCGTCGCCGCGCTCTCCCTCTCCCCCTGTAGGCACCATCAATAGAT1955320.268006536699332No Hit
TTGACGTACGCAGTTTTATCCGGTAAAGCGCTGTAGGCACCATCAATAGA1816950.24904081012614368No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGTAGGCACCATCAATAG1806250.24757421133787227No Hit
TTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAG1719770.23572080356126368No Hit
TCGGGACCGGGGTCCGGTGCGGAGAGCCGTTCTGTAGGCACCATCAATAG1719420.23567283070370346No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCCTGTAGGCACCATCAATAGA1713940.23492171281961674No Hit
GCCGCCGGTGAAATACCACTACTCTCACTGTAGGCACCATCAATAGATCG1691080.2317883998943939No Hit
TTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATC1670250.2289333295430207No Hit
CCGCCGCGACCGGCTCCGGGACGGCCGGGCTGTAGGCACCATCAATAGAT1666190.22837684439532147No Hit
TGGCCGCCGGTGAAATACCACTACTCTCCTGTAGGCACCATCAATAGATC1623820.2225693873243813No Hit
TCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAG1560940.21395072080040875No Hit
TCGGGACCGGGGTCCGGTGCGGAGAGCCGTCTGTAGGCACCATCAATAGA1560250.21385614573836134No Hit
TCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATAGA1551160.21261022209486724No Hit
TCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCCTGTAGGCACCATCA1539640.21103122975459745No Hit
TGGGCCGCCGGTGAAATACCACTACTCTCATCTGTAGGCACCATCAATAG1419940.19462451246898177No Hit
TACCCGTCGCCGCGCTCTCCCTCTCCCCCTGTAGGCACCATCAATAGATC1401280.1920668738344823No Hit
CTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCGG1394740.19117046672464164No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGTAGGCACCATCAATAG1378690.1889705685422345No Hit
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCTGTAGGCACCATCAATAGA1372260.18808923861619853No Hit
CCGGGGTCCGGTGCGGAGAGCCGTTCTGTAGGCACCATCAATAGATCGGA1330050.18230371199442877No Hit
TTTTCGCCGAATCCCGGGGCCGAGGAAGCCCTGTAGGCACCATCAATAGA1298200.17793818195644331No Hit
TTTTCGCCGAATCCCGGGGCCGAGGAACTGTAGGCACCATCAATAGATCG1277700.1751283431564841No Hit
GCTTTCGCCGAATCCCGGGGCCGAGGAAGCTGTAGGCACCATCAATAGAT1263600.17319572232334138No Hit
TTACCCGTCGCCGCGCTCTCCCTCTCCCCTGTAGGCACCATCAATAGATC1232600.16894669779657376No Hit
CGTTTTTTCACTGACCCGGTGAGGCGGGCTGTAGGCACCATCAATAGATC1202870.1648717462100963No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGTAGGCACCATCAATAGA1188190.16285962749871083No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATAGA1179340.16164659952897234No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATA1139960.15624896772690428No Hit
TCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCTGTAGGCACCATCAAT1139360.15616672854251523No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAG1129220.15477688632634026No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATAG1117080.15311291349553513No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAAT1107250.15176556152462786No Hit
TCGACGTACGCAGTTTTATCCGGTAAAGCCTGTAGGCACCATCAATAGAT1086080.1488638889687675No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCACTGTAGGCACCATCAATAG1085060.14872408235530613No Hit
TTACCCGTCGCCGCGCTCTCTCTCTCCCCCTGTAGGCACCATCAATAGAT1083270.14847873545521212No Hit
GGGGCCGCCGGTGAAATACCACTACTCTCACTGTAGGCACCATCAATAGA1077190.1476453783867364No Hit
ACGTACGCAGTTTTATCCGGTAAAGCGCTGTAGGCACCATCAATAGATCG1030240.14121016220829316No Hit
TGCCGCCGGTGAAATACCACTACTCTCACTGTAGGCACCATCAATAGATC1016110.13927342941593102No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAACTGTAGGCACCATCAATAGATCG1013440.13890746504539972No Hit
GCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT1013070.13885675088169316No Hit
TTTTCGCCGAATCCCGGGGCCGAGGAAGCCACTGTAGGCACCATCAATAG958950.13143877644980073No Hit
TGCGCCGCGACCGGCTCCGGGACGGCCGGCTGTAGGCACCATCAATAGAT933100.1278956382557058No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCCTGTAGGCACCATCAATAGATCGG932180.12776953817297593No Hit
TACCCGTCGCCGCGCTCTCCCTCTCCCCTGTAGGCACCATCAATAGATCG931510.12767770441707482No Hit
TTTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGAT920590.1261809512611941No Hit
TTGCGCCGCGACCGGCTCCGGGACGGCCGGCTGTAGGCACCATCAATAGA910210.1247582133712635No Hit
GTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATC908270.12449230667507223No Hit
TGTGGGGAAGGTGGCTCGGGGGGCTGTAGGCACCATCAATAGATCGGAAG862020.11815303621174955No Hit
ATGTACACGGAGTCGACCCAACGCGACTGTAGGCACCATCAATAGATCGG848620.11631636109372741No Hit
TTGGCCGCCGGTGAAATACCACTACTCTCACTGTAGGCACCATCAATAGA836750.11468939589589736No Hit
GGGCCGCCGGTGAAATACCACTACTCTCACTGTAGGCACCATCAATAGAT828950.1136202864988397No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAAT828660.11358053755971832No Hit
TTGGGGAAGGTGGCTCGGGGGGCTGTAGGCACCATCAATAGATCGGAAGA814850.111687665665697No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCCTGTAGGCACCATCAAT814600.11165339933886824No Hit
TGCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCTGTAGGCACCATCA786340.10777993375414394No Hit
CCTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCG775750.10632841214967717No Hit
GCCGCCGGTGAAATACCACTACTCTCATCTGTAGGCACCATCAATAGATC749470.10272633587343676No Hit
TACCCGTCGCCGCGCTCTCTCTCTCCCCCTGTAGGCACCATCAATAGATC742010.10170382868086622No Hit
ATTAGAGTGTTCAAAGCAGGCCCCTGTAGGCACCATCAATAGATCGGAAG737640.1011048532878993No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)