FastQCFastQC Report
Tue 27 Jun 2023
SRR989510.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR989510.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences91395377
Total Bases4.5 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA37741054.129426590143613No Hit
TTTTCGCCGAATCCCGGGGCCGAGGAAGCTGTAGGCACCATCAATAGATC14185531.5521058576080933No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA13171601.4411669859406566No Hit
CGTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGAT11156371.220671150576905No Hit
TTGCGCCGCGACCGGCTCCGGGACGGCCGGGCTGTAGGCACCATCAATAG10722751.1732267377156287No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGCTGTAGGCACCATCAATAGATC10400121.1379262651326445No Hit
TGCGCCGCGACCGGCTCCGGGACGGCCGGGCTGTAGGCACCATCAATAGA10315721.1286916623802536No Hit
TGGCCGCCGGTGAAATACCACTACTCTCACTGTAGGCACCATCAATAGAT8752280.9576283054229319No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA8239540.9015269995549118No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCTGTAGGCACCATCAATAGATCGGA8118290.8882604642026914No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA8033950.8790324263337739No Hit
TCGCCGCGACCGGCTCCGGGACGGCCGGGCTGTAGGCACCATCAATAGAT6910780.7561410901560153No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT6450980.7058321998059048No Hit
TTTTCGCCGAATCCCGGGGCCGAGGAACTGTAGGCACCATCAATAGATCG6377990.6978460190606797No Hit
TTGACGTACGCAGTTTTATCCGGTAAAGCCTGTAGGCACCATCAATAGAT5443610.5956110887315449No Hit
GCGCCGCGACCGGCTCCGGGACGGCCGGGCTGTAGGCACCATCAATAGAT5431100.5942423105273695No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAG5279220.5776244021620481No Hit
TTTTCGCCGAATCCCGGGGCCGAGGAAGCCTGTAGGCACCATCAATAGAT5218470.5709774576453687No Hit
ACGTACGCAGTTTTATCCGGTAAAGCCTGTAGGCACCATCAATAGATCGG4986740.5456227835243789No Hit
TCGTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGA4944560.5410076704426746No Hit
TCTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCG4878780.5338103698614866No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCTGTAGGCACCATCAATAGAT4804010.5256294309065545No Hit
GCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA4523190.49490358795718953No Hit
TTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAG4424160.484068247784568No Hit
TTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATC3832050.4192826952286657No Hit
TGTGCGCCGCGACCGGCTCCGGGACGGCCGGGCTGTAGGCACCATCAATA3812200.41711081294626096No Hit
TGCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCA3796240.4153645539423728No Hit
TGGCCGCCGGTGAAATACCACTACTCTCATCTGTAGGCACCATCAATAGA3684210.403106822350544No Hit
GCCGCCGGTGAAATACCACTACTCTCACTGTAGGCACCATCAATAGATCG3413380.3734740324994775No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAAT3321030.36336958268687924No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAACTGTAGGCACCATCAATAGATCG3318180.36305775072189916No Hit
TCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATAGA3001790.32844002602013445No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAG2981480.32621781296443475No Hit
TGTGGGAAATGTGGCGTACGGAAGACCCACTCCCCGGCGCCTGTAGGCAC2961030.3239802818472974No Hit
CTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCGG2809820.30743568134742744No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGTAGGCACCATCAATAG2808930.307338302242574No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGTAGGCACCATCAATAG2778870.30404929562246896No Hit
TTTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGAT2735490.29930288487129936No Hit
TCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCTGTAGGCACCATCAA2705940.296069679760717No Hit
ATGTACACGGAGTCGACCCAACGCGACTGTAGGCACCATCAATAGATCGG2676800.29288133468720196No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGTAGGCACCATCAA2665340.29162744194380863No Hit
TTTTCGCCGAATCCCGGGGCCGAGGAAGCCCTGTAGGCACCATCAATAGA2660490.29109678052971977No Hit
CGTTTTTTCACTGACCCGGTGAGGCGGGCTGTAGGCACCATCAATAGATC2620140.28668189639395No Hit
TCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCCTGTAGGCACCATCA2546560.2786311609612377No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAAT2526640.27645161964811416No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCCTGTAGGCACCATCAATAGA2294910.2510969455271244No Hit
CCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT2277580.24920078835059678No Hit
TCGGGACCGGGGTCCGGTGCGGAGAGCCGTCTGTAGGCACCATCAATAGA2252790.24648839732889335No Hit
TCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAG2249570.2461360819158282No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGTAGGCACCATCAATAGA2009750.2198962426731934No Hit
CCTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCG1980680.21671555663039715No Hit
TTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATCG1954480.21384889084707207No Hit
TTGACGTACGCAGTTTTATCCGGTAAAGCGCTGTAGGCACCATCAATAGA1940100.21227550710797988No Hit
TCGGGACCGGGGTCCGGTGCGGAGAGCCGTTCTGTAGGCACCATCAATAG1897840.2076516408483112No Hit
TTGCGCCGCGACCGGCTCCGGGACGGCCGGCTGTAGGCACCATCAATAGA1855230.20298947943504847No Hit
ATGTACACGGAGTCGAGCTCAACCCGCAACGCGACTGTAGGCACCATCAA1816760.19878029498144092No Hit
TGCCGCCGGTGAAATACCACTACTCTCACTGTAGGCACCATCAATAGATC1813940.19847174545819754No Hit
TTTTCGCCGAATCCCGGGGCCGAGGAAGCCACTGTAGGCACCATCAATAG1800430.19699355252946765No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATCA1771400.1938172430756536No Hit
TGGCCGCCGGTGAAATACCACTACTCTCCTGTAGGCACCATCAATAGATC1745760.19101184953807893No Hit
TGGGCCGCCGGTGAAATACCACTACTCTCACTGTAGGCACCATCAATAGA1744750.190901340666279No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1661060.18174442236832178Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TTTTCGCCGAATCCCGGGGCCGAGGACTGTAGGCACCATCAATAGATCGG1638580.17928477936033899No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAAT1636680.17907689138368563No Hit
TCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAA1630040.17835037761264447No Hit
GTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATC1581770.17306892885840386No Hit
ACGTACGCAGTTTTATCCGGTAAAGCGCTGTAGGCACCATCAATAGATCG1575560.17238946341892106No Hit
GCCGCCGGTGAAATACCACTACTCTCATCTGTAGGCACCATCAATAGATC1524890.16684541932574992No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATAGA1517440.1660302796278197No Hit
TGCGCCGCGACCGGCTCCGGGACGGCCGGCTGTAGGCACCATCAATAGAT1498650.1639743769534426No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACACTGTAGGCACCATCA1475180.161406413368151No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCCTGTAGGCACCATCAAT1448970.1585386534375803No Hit
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCTGTAGGCACCATCAATAGA1440150.15757361556701058No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGTAGGCACCATCA1419690.15533499030262768No Hit
TCGTTTTTTCACTGACCCGGTGAGGCGGGCTGTAGGCACCATCAATAGAT1406660.1539093164416839No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCACTGTAGGCACCATCAATAG1393710.15249239575870452No Hit
TGCCGCCTGGATACCGCAGCTAGGAATAATGGAACTGTAGGCACCATCAA1382500.15126585669644976No Hit
TCTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCTGTAGGCACCATCAA1362660.1490950685612906No Hit
GCTTTCGCCGAATCCCGGGGCCGAGGAAGCTGTAGGCACCATCAATAGAT1269290.1388790157296468No Hit
TCGACGTACGCAGTTTTATCCGGTAAAGCCTGTAGGCACCATCAATAGAT1261540.13803105161435025No Hit
CTTTCGCCGAATCCCGGGGCCGAGGACTGTAGGCACCATCAATAGATCGG1250250.13679575937413116No Hit
TGTCGCGGCGTGGGAAATGTGGCGTACTGTAGGCACCATCAATAGATCGG1231210.13471250301861548No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATAG1230660.13465232492011056No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCATCATCAATA1158490.12675586424901994No Hit
TCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCTGTAGGCACCATCAAT1154290.12629632240589148No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAG1150980.12593415966761645No Hit
TCTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCCTGTAGGCACCATCA1119940.12253792661744807No Hit
TGCTTTCGCCGAATCCCGGGGCCGAGGAAGCTGTAGGCACCATCAATAGA1100460.12040652778312846No Hit
TGGCGGCGTCCGGTGAGCTCTCGCTGGCCCTGTAGGCACCATCAATAGAT1097290.12005968310629103No Hit
TCAACTTCTTAGAGGGACAAGTGGCGCTGTAGGCACCATCAATAGATCGG1096830.120009352332996No Hit
ACTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA1093340.11962749494430118No Hit
TTTCGCCGAATCCCGGGGCCGAGGAAGCTGTAGGCACCATCAATAGATCG1076300.11776306803789431No Hit
TGTGGGAAATGTGGCGTACGGAAGACCCACTCCCCGGCGCTGTAGGCACC1070340.11711095627955012No Hit
TGTGCGCCGCGACCGGCTCCGGGACGGCCGGCTGTAGGCACCATCAATAG1062730.11627831022569118No Hit
TCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCCCTGTAGGCACCATC1036550.11341383273685712No Hit
TTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGCTGTAGGCACCATCAATA1022130.11183607240878278No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAG1016450.11121459677331381No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGTAGGCACCATCAATAGA1013260.1108655638019853No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCCTGTAGGCACCATCAAT1004390.10989505519518783No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCTGTAGGCACCATCAAT998610.10926263808726341No Hit
CCGCCGCGACCGGCTCCGGGACGGCCGGGCTGTAGGCACCATCAATAGAT982700.10752184981960301No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCCTGTAGGCACCATCAA957430.10475693973011349No Hit
TGCCGCCGGTGAAATACCACTACTCTCATCTGTAGGCACCATCAATAGAT921320.10080597402645432No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)