FastQCFastQC Report
Tue 27 Jun 2023
SRR989513.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR989513.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences61868259
Total Bases3 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA39162586.329995482821005No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA21190503.4251004218495953No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA16823802.719294234544405No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAG15562712.515459502424337No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAG8609191.3915358439292755No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT7082341.1447453208599259No Hit
TGCGCCGCGACCGGCTCCGGGACGGCCGGGCTGTAGGCACCATCAATAGA6778651.0956587609811357No Hit
TCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAG6284271.0157502573330857No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATAGA6006040.9707788932609207No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA5743240.9283015382734464No Hit
CGTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGAT4807840.7771093089915461No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAG4748400.7675017976503913No Hit
TCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATAGA4447720.7189017554219523No Hit
TGCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCA4189190.677114576636139No Hit
GCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA3964810.6408471911259052No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATCA3641670.5886168544034833No Hit
TGGCCGCCGGTGAAATACCACTACTCTCACTGTAGGCACCATCAATAGAT3543070.5726797645946364No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATA3480970.5626423074229387No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGCTGTAGGCACCATCAATAGATC3048570.4927518648940808No Hit
CTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCGG3023720.488735265687693No Hit
TTGCGCCGCGACCGGCTCCGGGACGGCCGGGCTGTAGGCACCATCAATAG2979250.4815474118966237No Hit
TTTTCGCCGAATCCCGGGGCCGAGGAAGCTGTAGGCACCATCAATAGATC2965770.47936858866515053No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAAT2901330.4689529084695918No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGTAGGCACCATCAATAG2802220.4529333854375957No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGTAGGCACCATCAATAGA2610170.42189161973993805No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAAT2454790.39677696442047933No Hit
TGTGCGCCGCGACCGGCTCCGGGACGGCCGGGCTGTAGGCACCATCAATA2437340.3939564551186094No Hit
TCTCCCGGGGCTACGCCTGTCTGAGGGTCGCTCTGTAGGCACCATCAATA2419000.39099209176065547No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCTGTAGGCACCATCAATAGATCGGA2360980.3816141003741515No Hit
TCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCTGTAGGCACCATCAA2326460.37603450260334625No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAGA2312700.3738104219160264No Hit
TCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCCTGTAGGCACCATCA2275550.3678057273278047No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATAG2269560.36683754104022875No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGTAGGCACCATCAA2207310.3567758388028989No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAG2041010.32989614270542184No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCTGTAGGCACCATCAATAGAT2026600.3275670000670295No Hit
GCGCCGCGACCGGCTCCGGGACGGCCGGGCTGTAGGCACCATCAATAGAT1964990.31760874344306345No Hit
CCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT1937460.313158965730715No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAA1777960.2873783792752274No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGTAGGCACCATCA1731390.2798510945653085No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGA1720670.2781183805414663No Hit
TTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAG1708230.2761076564317092No Hit
ACGTACGCAGTTTTATCCGGTAAAGCCTGTAGGCACCATCAATAGATCGG1707110.2759266266083227No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAGA1509310.24395546672810042No Hit
TGGCCGCCGGTGAAATACCACTACTCTCATCTGTAGGCACCATCAATAGA1367290.2210002385876092No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAAT1345550.21748632040866062No Hit
TCTCCCGGGGCTACGCCTGTCTGAGAGTCGCTCTGTAGGCACCATCAATA1320540.2134438597989318No Hit
TTTTCGCCGAATCCCGGGGCCGAGGAAGCCTGTAGGCACCATCAATAGAT1313790.2123528318454864No Hit
CCTCCCGGGGCTACGCCTGTCTGAGGGTCGCTCTGTAGGCACCATCAATA1305830.21106622702927522No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAAT1270000.20527488901861615No Hit
TCTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCG1248120.20173834211174424No Hit
TTACCCGTCGCCGCGCTCTCCCTCTCCCCCTGTAGGCACCATCAATAGAT1201390.19418519599848444No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCTGTAGGCACCATCAAT1194880.19313296015005044No Hit
ACTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA1188740.19214052879684235No Hit
TCGTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGA1174370.18981785150928523No Hit
TTGACGTACGCAGTTTTATCCGGTAAAGCCTGTAGGCACCATCAATAGAT1130000.18264616109530413No Hit
GCCGCCGGTGAAATACCACTACTCTCACTGTAGGCACCATCAATAGATCG1111150.17959936451420105No Hit
CGTGGGGGGCCCAAGTCCTTCTGATGGAGGCCCTGTAGGCACCATCAATA1046520.16915297390217493No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATTAATA939750.1518953361852319No Hit
CCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATA938570.15170460833559257No Hit
TGCCGCCTGGATACCGCAGCTAGGAATAATGGAACTGTAGGCACCATCAA931670.15058933531651505No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCATCATCAATA925440.14958235692392766No Hit
TCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCTGTAGGCACCATCAAT912040.14741646439412495No Hit
TTTTCGCCGAATCCCGGGGCCGAGGAAGCCCTGTAGGCACCATCAATAGA882520.14264503547772373No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATC855540.13828415633935973No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGTAGGCACCATCAATAG850350.1374452770684884No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATAGAT839890.1357545878250752No Hit
TTACCCGTCGCCGCGCTCTCCCTCTCCCCTGTAGGCACCATCAATAGATC839520.13569478332984933No Hit
TGACGTACGCAGTTTTATCCGGTAAAGCCTGTAGGCACCATCAATAGATC824810.13331715056019275No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCACTGTAGGCACCATCAATAG823140.1330472221628218No Hit
TGGGCCGCCGGTGAAATACCACTACTCTCACTGTAGGCACCATCAATAGA820360.1325978802797732No Hit
TTTTCGCCGAATCCCGGGGCCGAGGAAGCCACTGTAGGCACCATCAATAG814390.1316329266676148No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCCTGTAGGCACCATCAAT787460.12728012921779486No Hit
TACCCGTCGCCGCGCTCTCCCTCTCCCCCTGTAGGCACCATCAATAGATC771760.1247424790149663No Hit
TCGGGACCGGGGTCCGGTGCGGAGAGCCGTTCTGTAGGCACCATCAATAG756760.1223179724517543No Hit
ATGTACACGGAGTCGACCCAACGCGACTGTAGGCACCATCAATAGATCGG749280.12110895184556592No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCCTGTAGGCACCATCAA747880.1208826645663328No Hit
ACGTACGCAGTTTTATCCGGTAAAGCGCTGTAGGCACCATCAATAGATCG746960.12073396149712246No Hit
ACCCGTCGCCGCGCTCTCCCTCTCCCCCTGTAGGCACCATCAATAGATCG737630.1192259184148046No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACTATCAATA735820.11893336128951035No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATCAA734370.11869899232173318No Hit
CTAAACCATTCGTAGACGCCCTGCTTCTGTAGGCACCATCAATAGATCGG728560.11775990011291575No Hit
CCCGTCGCCGCGCTCTCCCTCTCCCCCTGTAGGCACCATCAATAGATCGG715710.11568290615709745No Hit
TTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATC715220.11560370560936585No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGAT709820.11473088324660954No Hit
CCTCCCGGGGCTACGCCTGTCTGAGAGTCGCTCTGTAGGCACCATCAATA706500.11419425912728529No Hit
GCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT703330.11368188007359314No Hit
GCTTTCGCCGAATCCCGGGGCCGAGGAAGCTGTAGGCACCATCAATAGAT691380.11175035651156758No Hit
TCGGGACCGGGGTCCGGTGCGGAGAGCCGTCTGTAGGCACCATCAATAGA688500.11128485125143088No Hit
TTTTCGCCGAATCCCGGGGCCGAGGAACTGTAGGCACCATCAATAGATCG684830.11069165531229835No Hit
TCGCCGCGACCGGCTCCGGGACGGCCGGGCTGTAGGCACCATCAATAGAT674040.10894762692449451No Hit
GCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCCTGTAGGCACCATCA671740.10857586925146867No Hit
GTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATC653690.10565837968707023No Hit
CGTTTTTTCACTGACCCGGTGAGGCGGGCTGTAGGCACCATCAATAGATC649860.10503932234459676No Hit
TGCGCCGCGACCGGCTCCGGGACGGCCGGCTGTAGGCACCATCAATAGAT640070.10345692772767373No Hit
ACCCGTCGCCGCGCTCTCCCTCTCCCCTGTAGGCACCATCAATAGATCGG622900.1006816758816504No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)