FastQCFastQC Report
Tue 27 Jun 2023
SRR989514.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR989514.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences60894776
Total Bases3 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA33813815.552826074932931No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA13749692.257942454702518No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA13056952.144182285849939No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAG12229852.0083578269505415No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATCA7074261.1617186998109656No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA6485831.0650880791482016No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAG5920120.9721884846082692No Hit
TCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAG5545240.910626553581542No Hit
TGCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCA5488720.9013449692302012No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATAGA5035260.8268788114106865No Hit
TCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATAGA4346640.7137952194782685No Hit
CGTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGAT4314780.7085632435859522No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGTAGGCACCATCAA4280820.7029864105255925No Hit
TGGCCGCCGGTGAAATACCACTACTCTCACTGTAGGCACCATCAATAGAT4265770.7005149341546145No Hit
TGCGCCGCGACCGGCTCCGGGACGGCCGGGCTGTAGGCACCATCAATAGA4200750.6898374993611931No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT3927350.6449403804359178No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGTAGGCACCATCA3764210.6181499050099142No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCTGTAGGCACCATCAATAGATCGGA3691130.6061488755620023No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATC3229140.5302819407694348No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAAT3203330.5260434819564818No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGTAGGCACCATCAATAG3118170.5120587027038247No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGTAGGCACCATCAATAGA3081890.5061008845816265No Hit
GCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA3030800.49771100233622667No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATA2832090.46507930335436326No Hit
CTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCGG2626460.43131121789494714No Hit
TTGCGCCGCGACCGGCTCCGGGACGGCCGGGCTGTAGGCACCATCAATAG2490660.40901045436147104No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATAG2301790.37799465753843975No Hit
TTTTCGCCGAATCCCGGGGCCGAGGAAGCTGTAGGCACCATCAATAGATC2219590.3644959626750249No Hit
TGGCCGCCGGTGAAATACCACTACTCTCATCTGTAGGCACCATCAATAGA2211870.36322820203821754No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAG2114730.34727609475072213No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGCTGTAGGCACCATCAATAGATC2004810.32922528526913375No Hit
TTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAG2002140.3287868240126214No Hit
TCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCCTGTAGGCACCATCA1984350.32586539114619617No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAAT1952210.32058743429814074No Hit
TGTGCGCCGCGACCGGCTCCGGGACGGCCGGGCTGTAGGCACCATCAATA1945640.319508524015262No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAG1940810.31871535252876204No Hit
ACGTACGCAGTTTTATCCGGTAAAGCCTGTAGGCACCATCAATAGATCGG1890890.3105176049912721No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAGA1862670.3058833815235645No Hit
TTGACGTACGCAGTTTTATCCGGTAAAGCCTGTAGGCACCATCAATAGAT1722510.2828666288221505No Hit
TCTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCG1665150.27344710160359237No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCCTGTAGGCACCATCAA1635400.26856162505631026No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGTAGGCACCATCAATAG1497600.24593242612469748No Hit
GCGCCGCGACCGGCTCCGGGACGGCCGGGCTGTAGGCACCATCAATAGAT1461200.23995490187861107No Hit
TCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCTGTAGGCACCATCAA1456670.23921099570183166No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAAT1442760.23692672750779148No Hit
TCGTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGA1350910.2218433318483674No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCCTGTAGGCACCATCAAT1297800.2131217298508496No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCTGTAGGCACCATCAATAGAT1282020.2105303745595517No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGA1262740.20736425732151473No Hit
GCCGCCGGTGAAATACCACTACTCTCACTGTAGGCACCATCAATAGATCG1252910.20574999733967325No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACACTGTAGGCACCATCA1227310.2015460242435246No Hit
TGCCGCCTGGATACCGCAGCTAGGAATAATGGAACTGTAGGCACCATCAA1211680.19897930160708696No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAA1202940.19754403891722996No Hit
TCTCCCGGGGCTACGCCTGTCTGAGAGTCGCTCTGTAGGCACCATCAATA1173800.19275873516637945No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCTGTAGGCACCATCAAT1149600.18878466684892642No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCCTGTAGGCACCATCAAT1130710.1856825945135261No Hit
TGGGCCGCCGGTGAAATACCACTACTCTCACTGTAGGCACCATCAATAGA1065790.17502158149001157No Hit
ACTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA1058990.17390490113634707No Hit
TTACCCGTCGCCGCGCTCTCCCTCTCCCCCTGTAGGCACCATCAATAGAT987910.16223230708657174No Hit
CCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT967810.15893153133529878No Hit
TTTTCGCCGAATCCCGGGGCCGAGGAAGCCTGTAGGCACCATCAATAGAT937710.15398857859334272No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCCTGTAGGCACCATCAA920830.15121658383306968No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAAT905070.14862851289575316No Hit
TCGGGACCGGGGTCCGGTGCGGAGAGCCGTTCTGTAGGCACCATCAATAG902540.14821304211711034No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCATCATCAATA894700.14692557535641482No Hit
TCGGGACCGGGGTCCGGTGCGGAGAGCCGTCTGTAGGCACCATCAATAGA880210.14454606089691502No Hit
TCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCCCTGTAGGCACCATC872340.14325366760524746No Hit
CGTGGGGGGCCCAAGTCCTTCTGATGGAGGCCCTGTAGGCACCATCAATA855320.14045868236710488No Hit
TGGCCGCCGGTGAAATACCACTACTCTCCTGTAGGCACCATCAATAGATC851060.13975911496907387No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCACTGTAGGCACCATCAATAG838460.1376899719608132No Hit
TCTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCCTGTAGGCACCATCA808350.13274537704186645No Hit
CCTCCCGGGGCTACGCCTGTCTGAGGGTCGCTCTGTAGGCACCATCAATA805780.1323233375552609No Hit
TTTTCGCCGAATCCCGGGGCCGAGGAAGCCACTGTAGGCACCATCAATAG787570.12933293325522702No Hit
TTGACGTACGCAGTTTTATCCGGTAAAGCGCTGTAGGCACCATCAATAGA781180.12828358215818053No Hit
TTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATC779180.1279551467600439No Hit
GCCGCCGGTGAAATACCACTACTCTCATCTGTAGGCACCATCAATAGATC769770.12640985821181114No Hit
TTTTGGTGACTCTAGATACCCTCGGGCCGATCGCACTGTAGGCACCATCA766840.125928700353541No Hit
ATGTACACGGAGTCGACCCAACGCGACTGTAGGCACCATCAATAGATCGG757730.12443267711502871No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACACTGTAGGCACCATCAA733550.12046189315155703No Hit
CCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATA728890.11969663867389872No Hit
TTTTCGCCGAATCCCGGGGCCGAGGAAGCCCTGTAGGCACCATCAATAGA725680.11916949985988946No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGTAGGCACCATCAATA710940.1167489309756226No Hit
TTTTGGTGACTCTAGATACCCTCGGGCCGATCGCACCTGTAGGCACCATC686970.11281263272895528No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCCTGTAGGCACCATCAATAGA675970.11100623803920388No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATTAATA674410.11075005842865733No Hit
TGACGTACGCAGTTTTATCCGGTAAAGCCTGTAGGCACCATCAATAGATC642010.10542940497884418No Hit
TCGCCGCGACCGGCTCCGGGACGGCCGGGCTGTAGGCACCATCAATAGAT636920.10459353689058648No Hit
CGTTTTTTCACTGACCCGGTGAGGCGGGCTGTAGGCACCATCAATAGATC633990.10411237903231634No Hit
CTAAACCATTCGTAGACGCCCTGCTTCTGTAGGCACCATCAATAGATCGG633630.10405326066065175No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGAT630710.10357374497937227No Hit
ATGTACACGGAGTCGAGCTCAACCCGCAACGCGACTGTAGGCACCATCAA613180.10069500871470487No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)