Lister et al. 2024 (PRJNA1105042)
General Details
| Title | Translational control in the spinal cord regulates gene expression and pain hypersensitivity in the chronic phase of neuropathic pain |
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| Organism | |
| Number of Samples | 24 |
| Release Date | 2024/04/26 00:00 |
| Sequencing Types | |
| Protocol Details |
Study Links
| GWIPS-viz | Trips-Viz |
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Repository Details
| SRA | SRP504307 |
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| ENA | SRP504307 |
| GEO | |
| BioProject | PRJNA1105042 |
Publication
| Title | |
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| Authors | Lister KC,Wong C,Uttam S,Parisien M,Stecum P,Brown N,Cai W,Hooshmandi M,Gu N,Amiri M,Beaudry F,Jafarnejad SM,Tavares-Ferreira D,Inturi NN,Mazhar K,Zhao HT,Fitzsimmons B,Gkogkas CG,Sonenberg N,Price TJ,Diatchenko L,Atlasi Y,Mogil JS,Khoutorsky A |
| Journal | bioRxiv : the preprint server for biology |
| Publication Date | 2024 Jun 28 |
| Abstract | Sensitization of spinal nociceptive circuits plays a crucial role in neuropathic pain. This sensitization depends on new gene expression that is primarily regulated via transcriptional and translational control mechanisms. The relative roles of these mechanisms in regulating gene expression in the clinically relevant chronic phase of neuropathic pain are not well understood. Here, we show that changes in gene expression in the spinal cord during the chronic phase of neuropathic pain are substantially regulated at the translational level. Downregulating spinal translation at the chronic phase alleviated pain hypersensitivity. Cell-type-specific profiling revealed that spinal inhibitory neurons exhibited greater changes in translation after peripheral nerve injury compared to excitatory neurons. Notably, increasing translation selectively in all inhibitory neurons or parvalbumin-positive (PV + ) interneurons, but not excitatory neurons, promoted mechanical pain hypersensitivity. Furthermore, increasing translation in PV + neurons decreased their intrinsic excitability and spiking activity, whereas reducing translation in spinal PV + neurons prevented the nerve injury-induced decrease in excitability. Thus, translational control mechanisms in the spinal cord, particularly in inhibitory neurons, play a role in mediating neuropathic pain hypersensitivity. |
| PMC | PMC11230214 |
| PMID | 38979173 |
| DOI |
| Run Accession | Study Accession | Scientific Name | Cell Line | Library Type | Treatment | GWIPS-viz | Trips-Viz | Reads | BAM | BigWig (F) | BigWig (R) | ||
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| SRR28817149 | PRJNA1105042 | Mus musculus | all cells |  |
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| SRR28817147 | PRJNA1105042 | Mus musculus | all cells | |
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| SRR28817145 | PRJNA1105042 | Mus musculus | all cells | |
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| SRR28817143 | PRJNA1105042 | Mus musculus | all cells | |
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| SRR28817141 | PRJNA1105042 | Mus musculus | all cells | |
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| SRR28817139 | PRJNA1105042 | Mus musculus | all cells | |
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| SRR28817137 | PRJNA1105042 | Mus musculus | inhibitory neurons | |
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| SRR28817135 | PRJNA1105042 | Mus musculus | inhibitory neurons | |
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| SRR28817117 | PRJNA1105042 | Mus musculus | all cells | |
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| SRR28817114 | PRJNA1105042 | Mus musculus | all cells | |
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| SRR28817112 | PRJNA1105042 | Mus musculus | all cells | |
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| SRR28817111 | PRJNA1105042 | Mus musculus | excitatory neurons | |
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| SRR28817109 | PRJNA1105042 | Mus musculus | excitatory neurons | |
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| SRR28817107 | PRJNA1105042 | Mus musculus | excitatory neurons | |
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| SRR28817105 | PRJNA1105042 | Mus musculus | excitatory neurons | |
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| SRR28817103 | PRJNA1105042 | Mus musculus | excitatory neurons | |
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| SRR28817085 | PRJNA1105042 | Mus musculus | excitatory neurons | |
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| SRR28817083 | PRJNA1105042 | Mus musculus | excitatory neurons | |
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| SRR28817081 | PRJNA1105042 | Mus musculus | excitatory neurons | |
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| SRR28817079 | PRJNA1105042 | Mus musculus | excitatory neurons | |
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| SRR28817077 | PRJNA1105042 | Mus musculus | excitatory neurons | |
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| SRR28817075 | PRJNA1105042 | Mus musculus | excitatory neurons | |
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| SRR28817073 | PRJNA1105042 | Mus musculus | inhibitory neurons | |
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| SRR28817066 | PRJNA1105042 | Mus musculus | inhibitory neurons | |
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| Run Accession | Study Accession | Scientific Name | Cell Line | Library Type | Treatment | GWIPS-viz | Trips-Viz | Reads | BAM | BigWig (F) | BigWig (R) |
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