Datasets

Title	Cytosolic N6AMT1-dependent translation supports mitochondrial RNA processing [Ribo-seq]
Organism
Number of Samples	6
Release Date	2024/05/09 00:00
Sequencing Types
Protocol Details

Title
Authors	Foged MM, Recazens E, Chollet S, Lisci M, Allen GE, Zinshteyn B, Boutguetait D, Münch C, Mootha VK, Jourdain AA
Journal	Proceedings of the National Academy of Sciences of the United States of America
Publication Date	2024 Nov 19
Abstract	Mitochondrial biogenesis relies on both the nuclear and mitochondrial genomes, and imbalance in their expression can lead to inborn errors of metabolism, inflammation, and aging. Here, we investigate N6AMT1, a nucleo-cytosolic methyltransferase that exhibits genetic codependency with mitochondria. We determine transcriptional and translational profiles of N6AMT1 and report that it is required for the cytosolic translation of TRMT10C (MRPP1) and PRORP (MRPP3), two subunits of the mitochondrial RNAse P enzyme. In the absence of N6AMT1 , or when its catalytic activity is abolished, RNA processing within mitochondria is impaired, leading to the accumulation of unprocessed and double-stranded RNA, thus preventing mitochondrial protein synthesis and oxidative phosphorylation, and leading to an immune response. Our work sheds light on the function of N6AMT1 in protein synthesis and highlights a cytosolic program required for proper mitochondrial biogenesis.
PMC
PMID	39503847
DOI

Foged et al. 2024 (PRJNA1109711)