Castañeda et al. 2014 (PRJNA219480)

General Details

Title MAEL-dependent selective processing of pachytene piRNA precursors and translation of spermiogenic mRNAs
Organism
Number of Samples 4
Release Date 2013/09/18 00:00
Sequencing Types
Protocol Details

Study Links

Repository Details

SRA SRP030014
ENA SRP030014
GEO GSE50983
BioProject PRJNA219480

Publication

Title
Authors Castañeda J,Genzor P,van der Heijden GW,Sarkeshik A,Yates JR 3rd,Ingolia NT,Bortvin A
Journal The EMBO journal
Publication Date 2014 Sep 17
Abstract Pachytene piRNAs are a class of Piwi-interacting small RNAs abundant in spermatids of the adult mouse testis. They are processed from piRNA primary transcripts by a poorly understood mechanism and, unlike fetal transposon-derived piRNAs, lack complementary targets in the spermatid transcriptome. We report that immunopurified complexes of a conserved piRNA pathway protein Maelstrom (MAEL) are enriched in MIWI (Piwi partner of pachytene piRNAs), Tudor-domain proteins and processing intermediates of pachytene piRNA primary transcripts. We provide evidence of functional significance of these complexes in Mael129 knockout mice that exhibit spermiogenic arrest with acrosome and flagellum malformation. Mael129-null mutant testes possess low levels of piRNAs derived from MAEL-associated piRNA precursors and exhibit reduced translation of numerous spermiogenic mRNAs including those encoding acrosome and flagellum proteins. These translation defects in haploid round spermatids are likely indirect, as neither MAEL nor piRNA precursors associate with polyribosomes, and they may arise from an imbalance between pachytene piRNAs and MIWI. © 2014 The Authors.
PMC PMC4195769
PMID 25063675
DOI
Run Accession Study Accession Scientific Name Cell Line Library Type Treatment GWIPS-viz Trips-Viz Reads BAM BigWig (F) BigWig (R)
SRR989509 PRJNA219480 Mus musculus Ribo-Seq
SRR989510 PRJNA219480 Mus musculus Ribo-Seq
SRR989513 PRJNA219480 Mus musculus Ribo-Seq
SRR989514 PRJNA219480 Mus musculus Ribo-Seq
Run Accession Study Accession Scientific Name Cell Line Library Type Treatment GWIPS-viz Trips-Viz Reads BAM BigWig (F) BigWig (R)

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