Datasets

Title	An Integrated Approach Reveals Regulatory Controls on Bacterial Translation Elongation
Organism
Number of Samples	6
Release Date	2013/09/20 00:00
Sequencing Types
Protocol Details

Title
Authors	Subramaniam AR,Zid BM,O'Shea EK
Journal	Cell
Publication Date	2014 Nov 20
Abstract	Ribosomes elongate at a nonuniform rate during translation. Theoretical models and experiments disagree on the in vivo determinants of elongation rate and the mechanism by which elongation rate affects protein levels. To resolve this conflict, we measured transcriptome-wide ribosome occupancy under multiple conditions and used it to formulate a whole-cell model of translation in E. coli. Our model predicts that elongation rates at most codons during nutrient-rich growth are not limited by the intracellular concentrations of aminoacyl-tRNAs. However, elongation pausing during starvation for single amino acids is highly sensitive to the kinetics of tRNA aminoacylation. We further show that translation abortion upon pausing accounts for the observed ribosome occupancy along mRNAs during starvation. Abortion reduces global protein synthesis, but it enhances the translation of a subset of mRNAs. These results suggest a regulatory role for aminoacylation and abortion during stress, and our study provides an experimentally constrained framework for modeling translation.
PMC	PMC4243059
PMID	25416955
DOI

Subramaniam et al. 2014 (PRJNA219750)