Kronja et al. 2015 (PRJNA230057)
General Details
Title | Polysome profiling and ribosome footprinting of Drosophila mature oocyte and activated egg |
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Organism | |
Number of Samples | 12 |
Release Date | 2013/11/27 00:00 |
Sequencing Types | |
Protocol Details |
Study Links
GWIPS-viz | Trips-Viz |
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Repository Details
SRA | SRP033366 |
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ENA | SRP033366 |
GEO | GSE52799 |
BioProject | PRJNA230057 |
Publication
Title | |
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Authors | Kronja I, Yuan B, Eichhorn SW, Dzeyk K, Krijgsveld J, Bartel DP, Orr-Weaver TL |
Journal | Cell reports |
Publication Date | 2014 Jun 12 |
Abstract | The oocyte-to-embryo transition marks the onset of development. The initial phase of this profound change from the differentiated oocyte to the totipotent embryo occurs in the absence of both transcription and mRNA degradation. Here we combine global polysome profiling, ribosome-footprint profiling, and quantitative mass spectrometry in a comprehensive approach to delineate the translational and proteomic changes that occur during this important transition in Drosophila. Our results show that PNG kinase is a critical regulator of the extensive changes in the translatome, acting uniquely at this developmental window. Analysis of the proteome in png mutants provided insights into the contributions of translation to changes in protein levels, revealing a compensatory dynamic between translation and protein turnover during proteome remodeling at the return to totipotency. The proteome changes additionally suggested regulators of meiosis and early embryogenesis, including the conserved H3K4 demethylase LID, which we demonstrated is required during this period despite transcriptional inactivity. Copyright © 2014 The Authors. Published by Elsevier Inc. All rights reserved. |
PMC | PMC4143395 |
PMID | 24882012 |
DOI |
Run Accession | Study Accession | Scientific Name | Cell Line | Library Type | Treatment | GWIPS-viz | Trips-Viz | Reads | BAM | BigWig (F) | BigWig (R) | ||
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SRR1039767 | PRJNA230057 | Drosophila melanogaster | 0.0 | Ribo-Seq | 0.0 | ||||||||
SRR1039768 | PRJNA230057 | Drosophila melanogaster | 0.0 | Ribo-Seq | 0.0 | ||||||||
SRR1039769 | PRJNA230057 | Drosophila melanogaster | 0.0 | Ribo-Seq | 0.0 | ||||||||
SRR1039770 | PRJNA230057 | Drosophila melanogaster | 0.0 | Ribo-Seq | Cycloheximide | ||||||||
SRR1039771 | PRJNA230057 | Drosophila melanogaster | 0.0 | Ribo-Seq | Cycloheximide | ||||||||
SRR1039772 | PRJNA230057 | Drosophila melanogaster | 0.0 | Ribo-Seq | Cycloheximide | ||||||||
SRR1039722 | PRJNA230057 | Drosophila melanogaster | 0.0 | LSU | 0.0 | ||||||||
SRR1039729 | PRJNA230057 | Drosophila melanogaster | 0.0 | LSU | 0.0 | ||||||||
SRR1039736 | PRJNA230057 | Drosophila melanogaster | 0.0 | LSU | 0.0 | ||||||||
SRR1039743 | PRJNA230057 | Drosophila melanogaster | 0.0 | LSU | 0.0 | ||||||||
SRR1039750 | PRJNA230057 | Drosophila melanogaster | 0.0 | LSU | 0.0 | ||||||||
SRR1039757 | PRJNA230057 | Drosophila melanogaster | 0.0 | LSU | 0.0 | ||||||||
Run Accession | Study Accession | Scientific Name | Cell Line | Library Type | Treatment | GWIPS-viz | Trips-Viz | Reads | BAM | BigWig (F) | BigWig (R) |
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