Kronja et al. 2015 (PRJNA230057)

General Details

Title Polysome profiling and ribosome footprinting of Drosophila mature oocyte and activated egg
Organism
Number of Samples 12
Release Date 2013/11/27 00:00
Sequencing Types
Protocol Details

Study Links

Repository Details

SRA SRP033366
ENA SRP033366
GEO GSE52799
BioProject PRJNA230057

Publication

Title
Authors Kronja I, Yuan B, Eichhorn SW, Dzeyk K, Krijgsveld J, Bartel DP, Orr-Weaver TL
Journal Cell reports
Publication Date 2014 Jun 12
Abstract The oocyte-to-embryo transition marks the onset of development. The initial phase of this profound change from the differentiated oocyte to the totipotent embryo occurs in the absence of both transcription and mRNA degradation. Here we combine global polysome profiling, ribosome-footprint profiling, and quantitative mass spectrometry in a comprehensive approach to delineate the translational and proteomic changes that occur during this important transition in Drosophila. Our results show that PNG kinase is a critical regulator of the extensive changes in the translatome, acting uniquely at this developmental window. Analysis of the proteome in png mutants provided insights into the contributions of translation to changes in protein levels, revealing a compensatory dynamic between translation and protein turnover during proteome remodeling at the return to totipotency. The proteome changes additionally suggested regulators of meiosis and early embryogenesis, including the conserved H3K4 demethylase LID, which we demonstrated is required during this period despite transcriptional inactivity. Copyright © 2014 The Authors. Published by Elsevier Inc. All rights reserved.
PMC PMC4143395
PMID 24882012
DOI
Run Accession Study Accession Scientific Name Cell Line Library Type Treatment GWIPS-viz Trips-Viz Reads BAM BigWig (F) BigWig (R)
SRR1039767 PRJNA230057 Drosophila melanogaster 0.0 Ribo-Seq 0.0
SRR1039768 PRJNA230057 Drosophila melanogaster 0.0 Ribo-Seq 0.0
SRR1039769 PRJNA230057 Drosophila melanogaster 0.0 Ribo-Seq 0.0
SRR1039770 PRJNA230057 Drosophila melanogaster 0.0 Ribo-Seq Cycloheximide
SRR1039771 PRJNA230057 Drosophila melanogaster 0.0 Ribo-Seq Cycloheximide
SRR1039772 PRJNA230057 Drosophila melanogaster 0.0 Ribo-Seq Cycloheximide
SRR1039722 PRJNA230057 Drosophila melanogaster 0.0 LSU 0.0
SRR1039729 PRJNA230057 Drosophila melanogaster 0.0 LSU 0.0
SRR1039736 PRJNA230057 Drosophila melanogaster 0.0 LSU 0.0
SRR1039743 PRJNA230057 Drosophila melanogaster 0.0 LSU 0.0
SRR1039750 PRJNA230057 Drosophila melanogaster 0.0 LSU 0.0
SRR1039757 PRJNA230057 Drosophila melanogaster 0.0 LSU 0.0
Run Accession Study Accession Scientific Name Cell Line Library Type Treatment GWIPS-viz Trips-Viz Reads BAM BigWig (F) BigWig (R)

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