Datasets

Title	Absolute quantification of protein production reveals principles underlying protein synthesis rates
Organism
Number of Samples	8
Release Date	2014/01/02 00:00
Sequencing Types
Protocol Details

GWIPS-viz	Trips-Viz
	Visit Trips-Viz

Title
Authors	Li GW,Burkhardt D,Gross C,Weissman JS
Journal	Cell
Publication Date	2014 Apr 24
Abstract	Quantitative views of cellular functions require precise measures of rates of biomolecule production, especially proteins-the direct effectors of biological processes. Here, we present a genome-wide approach, based on ribosome profiling, for measuring absolute protein synthesis rates. The resultant E. coli data set transforms our understanding of the extent to which protein synthesis is precisely controlled to optimize function and efficiency. Members of multiprotein complexes are made in precise proportion to their stoichiometry, whereas components of functional modules are produced differentially according to their hierarchical role. Estimates of absolute protein abundance also reveal principles for optimizing design. These include how the level of different types of transcription factors is optimized for rapid response and how a metabolic pathway (methionine biosynthesis) balances production cost with activity requirements. Our studies reveal how general principles, important both for understanding natural systems and for synthesizing new ones, emerge from quantitative analyses of protein synthesis. Copyright © 2014 Elsevier Inc. All rights reserved.
PMC	PMC4006352
PMID	24766808
DOI

Li et al. 2014 (PRJNA232843)