Datasets

Title	Musashi proteins are post-transcriptional regulators of the epithelial-luminal cell state
Organism
Number of Samples	8
Release Date	2014/06/12 00:00
Sequencing Types
Protocol Details

Title
Authors	Katz Y,Li F,Lambert NJ,Sokol ES,Tam WL,Cheng AW,Airoldi EM,Lengner CJ,Gupta PB,Yu Z,Jaenisch R,Burge CB
Journal	eLife
Publication Date	2014 Nov 7
Abstract	The conserved Musashi (Msi) family of RNA binding proteins are expressed in stem/progenitor and cancer cells, but generally absent from differentiated cells, consistent with a role in cell state regulation. We found that Msi genes are rarely mutated but frequently overexpressed in human cancers and are associated with an epithelial-luminal cell state. Using ribosome profiling and RNA-seq analysis, we found that Msi proteins regulate translation of genes implicated in epithelial cell biology and epithelial-to-mesenchymal transition (EMT), and promote an epithelial splicing pattern. Overexpression of Msi proteins inhibited the translation of Jagged1, a factor required for EMT, and repressed EMT in cell culture and in mammary gland in vivo. Knockdown of Msis in epithelial cancer cells promoted loss of epithelial identity. Our results show that mammalian Msi proteins contribute to an epithelial gene expression program in neural and mammary cell types.
PMC	PMC4381951
PMID	25380226
DOI

Katz et al. 2015 (PRJNA252579)