Geula et al. 2014 (PRJNA262909)

General Details

Title m6A mRNA Methylation Facilitates Resolution of Naïve Pluripotency Towards Differentiation (Ribo-seq)
Organism
Number of Samples 4
Release Date 2014/10/02 00:00
Sequencing Types
Protocol Details

Study Links

Repository Details

SRA SRP048597
ENA SRP048597
GEO GSE61996
BioProject PRJNA262909

Publication

Title
Authors Geula S,Moshitch-Moshkovitz S,Dominissini D,Mansour AA,Kol N,Salmon-Divon M,Hershkovitz V,Peer E,Mor N,Manor YS,Ben-Haim MS,Eyal E,Yunger S,Pinto Y,Jaitin DA,Viukov S,Rais Y,Krupalnik V,Chomsky E,Zerbib M,Maza I,Rechavi Y,Massarwa R,Hanna S,Amit I,Levanon EY,Amariglio N,Stern-Ginossar N,Novershtern N,Rechavi G,Hanna JH
Journal Science (New York, N.Y.)
Publication Date 2015 Feb 27
Abstract Naïve and primed pluripotent states retain distinct molecular properties, yet limited knowledge exists on how their state transitions are regulated. Here, we identify Mettl3, an N(6)-methyladenosine (m(6)A) transferase, as a regulator for terminating murine naïve pluripotency. Mettl3 knockout preimplantation epiblasts and naïve embryonic stem cells are depleted for m(6)A in mRNAs, yet are viable. However, they fail to adequately terminate their naïve state and, subsequently, undergo aberrant and restricted lineage priming at the postimplantation stage, which leads to early embryonic lethality. m(6)A predominantly and directly reduces mRNA stability, including that of key naïve pluripotency-promoting transcripts. This study highlights a critical role for an mRNA epigenetic modification in vivo and identifies regulatory modules that functionally influence naïve and primed pluripotency in an opposing manner. Copyright © 2015, American Association for the Advancement of Science.
PMC
PMID 25569111
DOI
Run Accession Study Accession Scientific Name Cell Line Library Type Treatment GWIPS-viz Trips-Viz Reads BAM BigWig (F) BigWig (R)
SRR1596101 PRJNA262909 Mus musculus Embryonic Stem Cells Ribo-Seq
SRR1596102 PRJNA262909 Mus musculus Embroid bodies Ribo-Seq
SRR1596103 PRJNA262909 Mus musculus Embryonic Stem Cells Ribo-Seq
SRR1596104 PRJNA262909 Mus musculus Embroid bodies Ribo-Seq
Run Accession Study Accession Scientific Name Cell Line Library Type Treatment GWIPS-viz Trips-Viz Reads BAM BigWig (F) BigWig (R)

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