Geula et al. 2014 (PRJNA262909)
General Details
| Title | m6A mRNA Methylation Facilitates Resolution of Naïve Pluripotency Towards Differentiation (Ribo-seq) |
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| Organism | |
| Number of Samples | 4 |
| Release Date | 2014/10/02 00:00 |
| Sequencing Types | |
| Protocol Details |
Study Links
| GWIPS-viz | Trips-Viz |
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Repository Details
| SRA | SRP048597 |
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| ENA | SRP048597 |
| GEO | GSE61996 |
| BioProject | PRJNA262909 |
Publication
| Title | |
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| Authors | Geula S, Moshitch-Moshkovitz S, Dominissini D, Mansour AA, Kol N, Salmon-Divon M, Hershkovitz V, Peer E, Mor N, Manor YS, Ben-Haim MS, Eyal E, Yunger S, Pinto Y, Jaitin DA, Viukov S, Rais Y, Krupalnik V, Chomsky E, Zerbib M, Maza I, Rechavi Y, Massarwa R, Hanna S, Amit I, Levanon EY, Amariglio N, Stern-Ginossar N, Novershtern N, Rechavi G, Hanna JH |
| Journal | Science (New York, N.Y.) |
| Publication Date | 2015 Feb 27 |
| Abstract | Naïve and primed pluripotent states retain distinct molecular properties, yet limited knowledge exists on how their state transitions are regulated. Here, we identify Mettl3, an N(6)-methyladenosine (m(6)A) transferase, as a regulator for terminating murine naïve pluripotency. Mettl3 knockout preimplantation epiblasts and naïve embryonic stem cells are depleted for m(6)A in mRNAs, yet are viable. However, they fail to adequately terminate their naïve state and, subsequently, undergo aberrant and restricted lineage priming at the postimplantation stage, which leads to early embryonic lethality. m(6)A predominantly and directly reduces mRNA stability, including that of key naïve pluripotency-promoting transcripts. This study highlights a critical role for an mRNA epigenetic modification in vivo and identifies regulatory modules that functionally influence naïve and primed pluripotency in an opposing manner. Copyright © 2015, American Association for the Advancement of Science. |
| PMC | |
| PMID | 25569111 |
| DOI |
| Run Accession | Study Accession | Scientific Name | Cell Line | Library Type | Treatment | GWIPS-viz | Trips-Viz | Reads | BAM | BigWig (F) | BigWig (R) | ||
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| SRR1596101 | PRJNA262909 | Mus musculus | EBCs | Ribo-Seq | 0.0 |  |
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| SRR1596102 | PRJNA262909 | Mus musculus | EBs | Ribo-Seq | 0.0 | |
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| SRR1596103 | PRJNA262909 | Mus musculus | EBCs | Ribo-Seq | 0.0 | |
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| SRR1596104 | PRJNA262909 | Mus musculus | EBs | Ribo-Seq | 0.0 | |
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| Run Accession | Study Accession | Scientific Name | Cell Line | Library Type | Treatment | GWIPS-viz | Trips-Viz | Reads | BAM | BigWig (F) | BigWig (R) |
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