Datasets

Title	HuR- dependent regulation of mRNA splicing is essential for the B cell antibody response [Ribo-Seq]
Organism
Number of Samples	18
Release Date	2014/10/07 00:00
Sequencing Types
Protocol Details

Title
Authors	Diaz-Muñoz MD,Bell SE,Fairfax K,Monzon-Casanova E,Cunningham AF,Gonzalez-Porta M,Andrews SR,Bunik VI,Zarnack K,Curk T,Heggermont WA,Heymans S,Gibson GE,Kontoyiannis DL,Ule J,Turner M
Journal	Nature immunology
Publication Date	2015 Apr
Abstract	Post-transcriptional regulation of mRNA by the RNA-binding protein HuR (encoded by Elavl1) is required in B cells for the germinal center reaction and for the production of class-switched antibodies in response to thymus-independent antigens. Transcriptome-wide examination of RNA isoforms and their abundance and translation in HuR-deficient B cells, together with direct measurements of HuR-RNA interactions, revealed that HuR-dependent splicing of mRNA affected hundreds of transcripts, including that encoding dihydrolipoamide S-succinyltransferase (Dlst), a subunit of the 2-oxoglutarate dehydrogenase (α-KGDH) complex. In the absence of HuR, defective mitochondrial metabolism resulted in large amounts of reactive oxygen species and B cell death. Our study shows how post-transcriptional processes control the balance of energy metabolism required for the proliferation and differentiation of B cells.
PMC	PMC4479220
PMID	25706746
DOI

Diaz-Muñoz et al. 2015 (PRJNA263261)