Arnold et al. 2015 (PRJNA265851)

General Details

Title Functional characterization of C. elegans Y-box binding proteins reveals tissue-specific functions and a critical role in the formation of polysomes (ribosome)
Organism
Number of Samples 8
Release Date 2014/10/30 00:00
Sequencing Types
Protocol Details

Study Links

Repository Details

SRA SRP049414
ENA SRP049414
GEO GSE62859
BioProject PRJNA265851

Publication

Title
Authors Arnold A,Rahman MM,Lee MC,Muehlhaeusser S,Katic I,Gaidatzis D,Hess D,Scheckel C,Wright JE,Stetak A,Boag PR,Ciosk R
Journal Nucleic acids research
Publication Date 2014 Dec 1
Abstract The cold shock domain is one of the most highly conserved motifs between bacteria and higher eukaryotes. Y-box-binding proteins represent a subfamily of cold shock domain proteins with pleiotropic functions, ranging from transcription in the nucleus to translation in the cytoplasm. These proteins have been investigated in all major model organisms except Caenorhabditis elegans. In this study, we set out to fill this gap and present a functional characterization of CEYs, the C. elegans Y-box-binding proteins. We find that, similar to other organisms, CEYs are essential for proper gametogenesis. However, we also report a novel function of these proteins in the formation of large polysomes in the soma. In the absence of the somatic CEYs, polysomes are dramatically reduced with a simultaneous increase in monosomes and disomes, which, unexpectedly, has no obvious impact on animal biology. Because transcripts that are enriched in polysomes in wild-type animals tend to be less abundant in the absence of CEYs, our findings suggest that large polysomes might depend on transcript stabilization mediated by CEY proteins. © The Author(s) 2014. Published by Oxford University Press on behalf of Nucleic Acids Research.
PMC PMC4245946
PMID 25378320
DOI
Run Accession Study Accession Scientific Name Cell Line Library Type Treatment GWIPS-viz Trips-Viz Reads BAM BigWig (F) BigWig (R)
SRR1635380 PRJNA265851 Caenorhabditis elegans Ribo-Seq
SRR1635381 PRJNA265851 Caenorhabditis elegans Ribo-Seq
SRR1635382 PRJNA265851 Caenorhabditis elegans Ribo-Seq
SRR1635383 PRJNA265851 Caenorhabditis elegans Ribo-Seq
SRR1635384 PRJNA265851 Caenorhabditis elegans Ribo-Seq
SRR1635385 PRJNA265851 Caenorhabditis elegans Ribo-Seq
SRR1635386 PRJNA265851 Caenorhabditis elegans Ribo-Seq
SRR1635387 PRJNA265851 Caenorhabditis elegans Ribo-Seq
Run Accession Study Accession Scientific Name Cell Line Library Type Treatment GWIPS-viz Trips-Viz Reads BAM BigWig (F) BigWig (R)

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