Riemondy et al. 2015 (PRJNA275789)
General Details
| Title | MicroRNA-203 represses selection and expansion of oncogenic HRas transformed tumor initiating cells [seq] |
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| Organism | |
| Number of Samples | 4 |
| Release Date | 2015/02/18 00:00 |
| Sequencing Types | |
| Protocol Details |
Study Links
| GWIPS-viz | Trips-Viz |
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Repository Details
| SRA | SRP055150 |
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| ENA | SRP055150 |
| GEO | GSE66054 |
| BioProject | PRJNA275789 |
Publication
| Title | |
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| Authors | Riemondy K,Wang XJ,Torchia EC,Roop DR,Yi R |
| Journal | eLife |
| Publication Date | 2015 Jul 23 |
| Abstract | In many mouse models of skin cancer, only a few tumors typically form even though many cells competent for tumorigenesis receive the same oncogenic stimuli. These observations suggest an active selection process for tumor-initiating cells. Here, we use quantitative mRNA- and miR-Seq to determine the impact of Hras(G12V) on the transcriptome of keratinocytes. We discover that microRNA-203 is downregulated by Hras(G12V). Using a knockout mouse model, we demonstrate that loss of microRNA-203 promotes selection and expansion of tumor-initiating cells. Conversely, restoration of microRNA-203 using an inducible model potently inhibits proliferation of these cells. We comprehensively identify microRNA-203 targets required for Hras-initiated tumorigenesis. These targets include critical regulators of the Ras pathway and essential genes required for cell division. This study establishes a role for the loss of microRNA-203 in promoting selection and expansion of Hras mutated cells and identifies a mechanism through which microRNA-203 antagonizes Hras-mediated tumorigenesis. |
| PMC | PMC4536367 |
| PMID | 26203562 |
| DOI |
| Run Accession | Study Accession | Scientific Name | Cell Line | Library Type | Treatment | GWIPS-viz | Trips-Viz | Reads | BAM | BigWig (F) | BigWig (R) | ||
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| SRR1810487 | PRJNA275789 | Mus musculus | Primary Keratinocytes | Ribo-Seq | Cycloheximide |  |
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| SRR1810488 | PRJNA275789 | Mus musculus | Primary Keratinocytes | Ribo-Seq | Cycloheximide | |
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| SRR1810489 | PRJNA275789 | Mus musculus | Primary Keratinocytes | Ribo-Seq | Cycloheximide | |
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| SRR1810490 | PRJNA275789 | Mus musculus | Primary Keratinocytes | Ribo-Seq | Cycloheximide | |
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| Run Accession | Study Accession | Scientific Name | Cell Line | Library Type | Treatment | GWIPS-viz | Trips-Viz | Reads | BAM | BigWig (F) | BigWig (R) |
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