Riemondy et al. 2015 (PRJNA275789)

General Details

Title MicroRNA-203 represses selection and expansion of oncogenic HRas transformed tumor initiating cells [seq]
Organism
Number of Samples 4
Release Date 2015/02/18 00:00
Sequencing Types
Protocol Details

Study Links

Repository Details

SRA SRP055150
ENA SRP055150
GEO GSE66054
BioProject PRJNA275789

Publication

Title
Authors Riemondy K,Wang XJ,Torchia EC,Roop DR,Yi R
Journal eLife
Publication Date 2015 Jul 23
Abstract In many mouse models of skin cancer, only a few tumors typically form even though many cells competent for tumorigenesis receive the same oncogenic stimuli. These observations suggest an active selection process for tumor-initiating cells. Here, we use quantitative mRNA- and miR-Seq to determine the impact of Hras(G12V) on the transcriptome of keratinocytes. We discover that microRNA-203 is downregulated by Hras(G12V). Using a knockout mouse model, we demonstrate that loss of microRNA-203 promotes selection and expansion of tumor-initiating cells. Conversely, restoration of microRNA-203 using an inducible model potently inhibits proliferation of these cells. We comprehensively identify microRNA-203 targets required for Hras-initiated tumorigenesis. These targets include critical regulators of the Ras pathway and essential genes required for cell division. This study establishes a role for the loss of microRNA-203 in promoting selection and expansion of Hras mutated cells and identifies a mechanism through which microRNA-203 antagonizes Hras-mediated tumorigenesis.
PMC PMC4536367
PMID 26203562
DOI
Run Accession Study Accession Scientific Name Cell Line Library Type Treatment GWIPS-viz Trips-Viz Reads BAM BigWig (F) BigWig (R)
SRR1810487 PRJNA275789 Mus musculus Primary Keratinocytes Ribo-Seq Cycloheximide
SRR1810488 PRJNA275789 Mus musculus Primary Keratinocytes Ribo-Seq Cycloheximide
SRR1810489 PRJNA275789 Mus musculus Primary Keratinocytes Ribo-Seq Cycloheximide
SRR1810490 PRJNA275789 Mus musculus Primary Keratinocytes Ribo-Seq Cycloheximide
Run Accession Study Accession Scientific Name Cell Line Library Type Treatment GWIPS-viz Trips-Viz Reads BAM BigWig (F) BigWig (R)

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