Su et al. 2015 (PRJNA278009)
General Details
| Title | IFN-g Regulates mTORC1, Cellular Metabolism and mRNA Translation to Potentiate Inflammatory Macrophage Activation [RNA-Seq] |
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| Organism | |
| Number of Samples | 4 |
| Release Date | 2015/03/12 00:00 |
| Sequencing Types | |
| Protocol Details |
Study Links
| GWIPS-viz | Trips-Viz |
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| Visit GWIPS-viz | Visit Trips-Viz |
Repository Details
| SRA | SRP056098 |
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| ENA | SRP056098 |
| GEO | |
| BioProject | PRJNA278009 |
Publication
| Title | |
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| Authors | Su X,Yu Y,Zhong Y,Giannopoulou EG,Hu X,Liu H,Cross JR,Rätsch G,Rice CM,Ivashkiv LB |
| Journal | Nature immunology |
| Publication Date | 2015 Aug |
| Abstract | Interferon-γ (IFN-γ) primes macrophages for enhanced microbial killing and inflammatory activation by Toll-like receptors (TLRs), but little is known about the regulation of cell metabolism or mRNA translation during this priming. We found that IFN-γ regulated the metabolism and mRNA translation of human macrophages by targeting the kinases mTORC1 and MNK, both of which converge on the selective regulator of translation initiation eIF4E. Physiological downregulation of mTORC1 by IFN-γ was associated with autophagy and translational suppression of repressors of inflammation such as HES1. Genome-wide ribosome profiling in TLR2-stimulated macrophages showed that IFN-γ selectively modulated the macrophage translatome to promote inflammation, further reprogram metabolic pathways and modulate protein synthesis. These results show that IFN-γ-mediated metabolic reprogramming and translational regulation are key components of classical inflammatory macrophage activation. |
| PMC | PMC4509841 |
| PMID | 26147685 |
| DOI |
| Run Accession | Study Accession | Scientific Name | Cell Line | Library Type | Treatment | GWIPS-viz | Trips-Viz | Reads | BAM | BigWig (F) | BigWig (R) | ||
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| SRR1910731 | PRJNA278009 | Homo sapiens | macrophage | Ribo-Seq | Cycloheximide |  |
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| SRR1910732 | PRJNA278009 | Homo sapiens | macrophage | Ribo-Seq | Cycloheximide | |
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| SRR1910735 | PRJNA278009 | Homo sapiens | macrophage | Ribo-Seq | Cycloheximide | |
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| SRR1910736 | PRJNA278009 | Homo sapiens | macrophage | Ribo-Seq | Cycloheximide | |
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| Run Accession | Study Accession | Scientific Name | Cell Line | Library Type | Treatment | GWIPS-viz | Trips-Viz | Reads | BAM | BigWig (F) | BigWig (R) |
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