Schmidt et al. 2017 (PRJNA288089)

General Details

Title The Ski complex binds 80S ribosomes for 3’ to 5’ decay of mRNA
Organism
Number of Samples 2
Release Date 2015/06/25 00:00
Sequencing Types
Protocol Details

Study Links

Repository Details

SRA SRP059872
ENA SRP059872
GEO
BioProject PRJNA288089

Publication

Title
Authors Schmidt C,Kowalinski E,Shanmuganathan V,Defenouillère Q,Braunger K,Heuer A,Pech M,Namane A,Berninghausen O,Fromont-Racine M,Jacquier A,Conti E,Becker T,Beckmann R
Journal Science (New York, N.Y.)
Publication Date 2016 Dec 16
Abstract Ski2-Ski3-Ski8 (Ski) is a helicase complex functioning with the RNA-degrading exosome to mediate the 3'-5' messenger RNA (mRNA) decay in turnover and quality-control pathways. We report that the Ski complex directly associates with 80S ribosomes presenting a short mRNA 3' overhang. We determined the structure of an endogenous ribosome-Ski complex using cryo-electron microscopy (EM) with a local resolution of the Ski complex ranging from 4 angstroms (Å) in the core to about 10 Å for intrinsically flexible regions. Ribosome binding displaces the autoinhibitory domain of the Ski2 helicase, positioning it in an open conformation near the ribosomal mRNA entry tunnel. We observe that the mRNA 3' overhang is threaded directly from the small ribosomal subunit to the helicase channel of Ski2, primed for ongoing exosome-mediated 3'-5' degradation. Copyright © 2016, American Association for the Advancement of Science.
PMC
PMID 27980209
DOI
Run Accession Study Accession Scientific Name Cell Line Library Type Treatment GWIPS-viz Trips-Viz Reads BAM BigWig (F) BigWig (R)
SRR2078180 PRJNA288089 Saccharomyces cerevisiae BY4741 Ribo-Seq
SRR2078181 PRJNA288089 Saccharomyces cerevisiae BY4741 Ribo-Seq
Run Accession Study Accession Scientific Name Cell Line Library Type Treatment GWIPS-viz Trips-Viz Reads BAM BigWig (F) BigWig (R)

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