Blanco et al. 2016 (PRJNA292862)
General Details
| Title | Ribosome Profiling of mouse skin squamous tumours |
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| Organism | |
| Number of Samples | 6 |
| Release Date | 2015/08/14 00:00 |
| Sequencing Types | |
| Protocol Details |
Study Links
| GWIPS-viz | Trips-Viz |
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Repository Details
| SRA | SRP062409 |
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| ENA | SRP062409 |
| GEO | GSE72066 |
| BioProject | PRJNA292862 |
Publication
| Title | |
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| Authors | Blanco S, Bandiera R, Popis M, Hussain S, Lombard P, Aleksic J, Sajini A, Tanna H, Cortés-Garrido R, Gkatza N, Dietmann S, Frye M |
| Journal | Nature |
| Publication Date | 2016 Jun 16 |
| Abstract | Whether protein synthesis and cellular stress response pathways interact to control stem cell function is currently unknown. Here we show that mouse skin stem cells synthesize less protein than their immediate progenitors in vivo, even when forced to proliferate. Our analyses reveal that activation of stress response pathways drives both a global reduction of protein synthesis and altered translational programmes that together promote stem cell functions and tumorigenesis. Mechanistically, we show that inhibition of post-transcriptional cytosine-5 methylation locks tumour-initiating cells in this distinct translational inhibition programme. Paradoxically, this inhibition renders stem cells hypersensitive to cytotoxic stress, as tumour regeneration after treatment with 5-fluorouracil is blocked. Thus, stem cells must revoke translation inhibition pathways to regenerate a tissue or tumour. |
| PMC | PMC5040503 |
| PMID | 27306184 |
| DOI |
| Run Accession | Study Accession | Scientific Name | Cell Line | Library Type | Treatment | GWIPS-viz | Trips-Viz | Reads | BAM | BigWig (F) | BigWig (R) | ||
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| SRR2163140 | PRJNA292862 | Mus musculus | 0.0 | Ribo-Seq | 0.0 |  |
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| SRR2163141 | PRJNA292862 | Mus musculus | 0.0 | Ribo-Seq | 0.0 | |
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| SRR2163142 | PRJNA292862 | Mus musculus | 0.0 | Ribo-Seq | 0.0 | |
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| SRR2163143 | PRJNA292862 | Mus musculus | 0.0 | Ribo-Seq | 0.0 | |
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| SRR2163144 | PRJNA292862 | Mus musculus | 0.0 | Ribo-Seq | 0.0 | |
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| SRR2163145 | PRJNA292862 | Mus musculus | 0.0 | Ribo-Seq | 0.0 | |
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| Run Accession | Study Accession | Scientific Name | Cell Line | Library Type | Treatment | GWIPS-viz | Trips-Viz | Reads | BAM | BigWig (F) | BigWig (R) |
ⓘ For more Information on the columns shown here see: About