Blanco et al. 2016 (PRJNA292862)

General Details

Title Ribosome Profiling of mouse skin squamous tumours
Organism
Number of Samples 6
Release Date 2015/08/14 00:00
Sequencing Types
Protocol Details

Study Links

Repository Details

SRA SRP062409
ENA SRP062409
GEO
BioProject PRJNA292862

Publication

Title
Authors Blanco S,Bandiera R,Popis M,Hussain S,Lombard P,Aleksic J,Sajini A,Tanna H,Cortés-Garrido R,Gkatza N,Dietmann S,Frye M
Journal Nature
Publication Date 2016 Jun 16
Abstract Whether protein synthesis and cellular stress response pathways interact to control stem cell function is currently unknown. Here we show that mouse skin stem cells synthesize less protein than their immediate progenitors in vivo, even when forced to proliferate. Our analyses reveal that activation of stress response pathways drives both a global reduction of protein synthesis and altered translational programmes that together promote stem cell functions and tumorigenesis. Mechanistically, we show that inhibition of post-transcriptional cytosine-5 methylation locks tumour-initiating cells in this distinct translational inhibition programme. Paradoxically, this inhibition renders stem cells hypersensitive to cytotoxic stress, as tumour regeneration after treatment with 5-fluorouracil is blocked. Thus, stem cells must revoke translation inhibition pathways to regenerate a tissue or tumour.
PMC PMC5040503
PMID 27306184
DOI
Run Accession Study Accession Scientific Name Cell Line Library Type Treatment GWIPS-viz Trips-Viz Reads BAM BigWig (F) BigWig (R)
SRR2163140 PRJNA292862 Mus musculus Ribo-Seq
SRR2163141 PRJNA292862 Mus musculus Ribo-Seq
SRR2163142 PRJNA292862 Mus musculus Ribo-Seq
SRR2163143 PRJNA292862 Mus musculus Ribo-Seq
SRR2163144 PRJNA292862 Mus musculus Ribo-Seq
SRR2163145 PRJNA292862 Mus musculus Ribo-Seq
Run Accession Study Accession Scientific Name Cell Line Library Type Treatment GWIPS-viz Trips-Viz Reads BAM BigWig (F) BigWig (R)

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