Chartron et al. 2016 (PRJNA300274)

General Details

Title Cotranslational signal independent SRP preloading during membrane targeting
Organism
Number of Samples 4
Release Date 2015/10/27 00:00
Sequencing Types
Protocol Details

Study Links

Repository Details

SRA SRP065322
ENA SRP065322
GEO GSE74393
BioProject PRJNA300274

Publication

Title
Authors Chartron JW,Hunt KC,Frydman J
Journal Nature
Publication Date 2016 Aug 11
Abstract Ribosome-associated factors must properly decode the limited information available in nascent polypeptides to direct them to their correct cellular fate. It is unclear how the low complexity information exposed by the nascent chain suffices for accurate recognition by the many factors competing for the limited surface near the ribosomal exit site. Questions remain even for the well-studied cotranslational targeting cycle to the endoplasmic reticulum, involving recognition of linear hydrophobic signal sequences or transmembrane domains by the signal recognition particle (SRP). Notably, the SRP has low abundance relative to the large number of ribosome-nascent-chain complexes (RNCs), yet it accurately selects those destined for the endoplasmic reticulum. Despite their overlapping specificities, the SRP and the cotranslationally acting Hsp70 display precise mutually exclusive selectivity in vivo for their cognate RNCs. To understand cotranslational nascent chain recognition in vivo, here we investigate the cotranslational membrane-targeting cycle using ribosome profiling in yeast cells coupled with biochemical fractionation of ribosome populations. We show that the SRP preferentially binds secretory RNCs before their targeting signals are translated. Non-coding mRNA elements can promote this signal-independent pre-recruitment of SRP. Our study defines the complex kinetic interaction between elongation in the cytosol and determinants in the polypeptide and mRNA that modulate SRP–substrate selection and membrane targeting.
PMC PMC5120976
PMID 27487213
DOI
Run Accession Study Accession Scientific Name Cell Line Library Type Treatment GWIPS-viz Trips-Viz Reads BAM BigWig (F) BigWig (R)
SRR2829326 PRJNA300274 Saccharomyces cerevisiae Ribo-Seq Cycloheximide
SRR2829327 PRJNA300274 Saccharomyces cerevisiae Ribo-Seq Cycloheximide
SRR2829332 PRJNA300274 Saccharomyces cerevisiae Ribo-Seq Cycloheximide
SRR2829333 PRJNA300274 Saccharomyces cerevisiae Ribo-Seq Cycloheximide
Run Accession Study Accession Scientific Name Cell Line Library Type Treatment GWIPS-viz Trips-Viz Reads BAM BigWig (F) BigWig (R)

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