Hornstein et al. 2016 (PRJNA312799)
General Details
| Title | Ligation-Free Ribosome Profiling Reveals Cell Type-Specific Regulation of Protein Synthesis in the Brain |
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| Organism | |
| Number of Samples | 12 |
| Release Date | 2016/02/22 00:00 |
| Sequencing Types | |
| Protocol Details |
Study Links
| GWIPS-viz | Trips-Viz |
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Repository Details
| SRA | SRP070655 |
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| ENA | SRP070655 |
| GEO | GSE78163 |
| BioProject | PRJNA312799 |
Publication
| Title | |
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| Authors | Hornstein N, Torres D, Das Sharma S, Tang G, Canoll P, Sims PA |
| Journal | Genome biology |
| Publication Date | 2016 Jul 5 |
| Abstract | Ribosome profiling has emerged as a powerful tool for genome-wide measurements of translation, but library construction requires multiple ligation steps and remains cumbersome relative to more conventional deep-sequencing experiments. We report a new, ligation-free approach to ribosome profiling that does not require ligation. Library construction for ligation-free ribosome profiling can be completed in one day with as little as 1 ng of purified RNA footprints. We apply ligation-free ribosome profiling to mouse brain tissue to identify new patterns of cell type-specific translation and test its ability to identify translational targets of mTOR signaling in the brain. |
| PMC | PMC4934013 |
| PMID | 27380875 |
| DOI |
| Run Accession | Study Accession | Scientific Name | Cell Line | Library Type | Treatment | GWIPS-viz | Trips-Viz | Reads | BAM | BigWig (F) | BigWig (R) | ||
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| SRR3182046 | PRJNA312799 | Mus musculus | 0.0 | RNA-Seq | 0.0 |  |
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| SRR3182047 | PRJNA312799 | Mus musculus | 0.0 | RiboTag | 0.0 | |
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| SRR3182048 | PRJNA312799 | Mus musculus | 0.0 | RNA-Seq | 0.0 | |
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| SRR3182049 | PRJNA312799 | Mus musculus | 0.0 | RiboTag | 0.0 | |
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| SRR3182050 | PRJNA312799 | Mus musculus | 0.0 | RNA-Seq | 0.0 | |
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| SRR3182051 | PRJNA312799 | Mus musculus | 0.0 | RNA-Seq | 0.0 | |
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| SRR3182052 | PRJNA312799 | Mus musculus | 0.0 | RNA-Seq | 0.0 | |
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| SRR3182053 | PRJNA312799 | Mus musculus | 0.0 | RNA-Seq | 0.0 | |
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| SRR3182054 | PRJNA312799 | Mus musculus | 0.0 | RNA-Seq | 0.0 | |
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| SRR3182055 | PRJNA312799 | Mus musculus | 0.0 | RNA-Seq | 0.0 | |
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| SRR3182056 | PRJNA312799 | Mus musculus | 0.0 | RNA-Seq | 0.0 | |
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| SRR3182057 | PRJNA312799 | Mus musculus | 0.0 | RNA-Seq | 0.0 | |
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| Run Accession | Study Accession | Scientific Name | Cell Line | Library Type | Treatment | GWIPS-viz | Trips-Viz | Reads | BAM | BigWig (F) | BigWig (R) |
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