Datasets

Title	MYC dependent mRNA translation shapes gene expression and cell biology
Organism
Number of Samples	12
Release Date	2016/04/03 00:00
Sequencing Types
Protocol Details

Title
Authors	Singh K,Lin J,Zhong Y,Burčul A,Mohan P,Jiang M,Sun L,Yong-Gonzalez V,Viale A,Cross JR,Hendrickson RC,Rätsch G,Ouyang Z,Wendel HG
Journal	The Journal of experimental medicine
Publication Date	2019 Jul 1
Abstract	The oncogenic c-MYC (MYC) transcription factor has broad effects on gene expression and cell behavior. We show that MYC alters the efficiency and quality of mRNA translation into functional proteins. Specifically, MYC drives the translation of most protein components of the electron transport chain in lymphoma cells, and many of these effects are independent from proliferation. Specific interactions of MYC-sensitive RNA-binding proteins (e.g., SRSF1/RBM42) with 5'UTR sequence motifs mediate many of these changes. Moreover, we observe a striking shift in translation initiation site usage. For example, in low-MYC conditions, lymphoma cells initiate translation of the CD19 mRNA from a site in exon 5. This results in the truncation of all extracellular CD19 domains and facilitates escape from CD19-directed CAR-T cell therapy. Together, our findings reveal MYC effects on the translation of key metabolic enzymes and immune receptors in lymphoma cells. © 2019 Singh et al.
PMC	PMC6605752
PMID	31142587
DOI

Singh et al. 2019 (PRJNA317266)