Blank et al. 2017 (PRJNA323467)

General Details

Title Ribosome profiling of synchronous, non-arrested yeast cells identifies translational control of lipid biosynthesis enzymes in the cell cycle
Organism
Number of Samples 48
Release Date 2016/05/26 00:00
Sequencing Types
Protocol Details

Study Links

Repository Details

SRA SRP075766
ENA SRP075766
GEO GSE81932
BioProject PRJNA323467

Publication

Title
Authors Blank HM, Perez R, He C, Maitra N, Metz R, Hill J, Lin Y, Johnson CD, Bankaitis VA, Kennedy BK, Aramayo R, Polymenis M
Journal The EMBO journal
Publication Date 2017 Feb 15
Abstract Translational control during cell division determines when cells start a new cell cycle, how fast they complete it, the number of successive divisions, and how cells coordinate proliferation with available nutrients. The translational efficiencies of mRNAs in cells progressing synchronously through the mitotic cell cycle, while preserving the coupling of cell division with cell growth, remain uninvestigated. We now report comprehensive ribosome profiling of a yeast cell size series from the time of cell birth, to identify mRNAs under periodic translational control. The data reveal coordinate translational activation of mRNAs encoding lipogenic enzymes late in the cell cycle including Acc1p, the rate-limiting enzyme acetyl-CoA carboxylase. An upstream open reading frame (uORF) confers the translational control of ACC1 and adjusts Acc1p protein levels in different nutrients. The ACC1 uORF is relevant for cell division because its ablation delays cell cycle progression, reduces cell size, and suppresses the replicative longevity of cells lacking the Sch9p protein kinase regulator of ribosome biogenesis. These findings establish an unexpected relationship between lipogenesis and protein synthesis in mitotic cell divisions. © 2017 The Authors.
PMC PMC5694946
PMID 28057705
DOI
Run Accession Study Accession Scientific Name Cell Line Library Type Treatment GWIPS-viz Trips-Viz Reads BAM BigWig (F) BigWig (R)
SRR3590722 PRJNA323467 Saccharomyces cerevisiae BY4743 Ribo-Seq 0.0
SRR3590723 PRJNA323467 Saccharomyces cerevisiae BY4743 Ribo-Seq 0.0
SRR3590724 PRJNA323467 Saccharomyces cerevisiae BY4743 Ribo-Seq 0.0
SRR3590725 PRJNA323467 Saccharomyces cerevisiae BY4743 Ribo-Seq 0.0
SRR3590726 PRJNA323467 Saccharomyces cerevisiae BY4743 Ribo-Seq 0.0
SRR3590727 PRJNA323467 Saccharomyces cerevisiae BY4743 Ribo-Seq 0.0
SRR3590728 PRJNA323467 Saccharomyces cerevisiae BY4743 Ribo-Seq 0.0
SRR3590729 PRJNA323467 Saccharomyces cerevisiae BY4743 Ribo-Seq 0.0
SRR3590730 PRJNA323467 Saccharomyces cerevisiae BY4743 Ribo-Seq 0.0
SRR3590731 PRJNA323467 Saccharomyces cerevisiae BY4743 Ribo-Seq 0.0
SRR3590732 PRJNA323467 Saccharomyces cerevisiae BY4743 Ribo-Seq 0.0
SRR3590733 PRJNA323467 Saccharomyces cerevisiae BY4743 Ribo-Seq 0.0
SRR3590734 PRJNA323467 Saccharomyces cerevisiae BY4743 Ribo-Seq 0.0
SRR3590735 PRJNA323467 Saccharomyces cerevisiae BY4743 Ribo-Seq 0.0
SRR3590736 PRJNA323467 Saccharomyces cerevisiae BY4743 Ribo-Seq 0.0
SRR3590737 PRJNA323467 Saccharomyces cerevisiae BY4743 Ribo-Seq 0.0
SRR3590738 PRJNA323467 Saccharomyces cerevisiae BY4743 Ribo-Seq 0.0
SRR3590739 PRJNA323467 Saccharomyces cerevisiae BY4743 Ribo-Seq 0.0
SRR3590740 PRJNA323467 Saccharomyces cerevisiae BY4743 Ribo-Seq 0.0
SRR3590741 PRJNA323467 Saccharomyces cerevisiae BY4743 Ribo-Seq 0.0
SRR3590742 PRJNA323467 Saccharomyces cerevisiae BY4743 Ribo-Seq 0.0
SRR3590743 PRJNA323467 Saccharomyces cerevisiae BY4743 Ribo-Seq 0.0
SRR3590744 PRJNA323467 Saccharomyces cerevisiae BY4743 Ribo-Seq 0.0
SRR3590745 PRJNA323467 Saccharomyces cerevisiae BY4743 Ribo-Seq 0.0
SRR3590746 PRJNA323467 Saccharomyces cerevisiae BY4743 Ribo-Seq 0.0
SRR3590747 PRJNA323467 Saccharomyces cerevisiae BY4743 Ribo-Seq 0.0
SRR3590748 PRJNA323467 Saccharomyces cerevisiae BY4743 Ribo-Seq 0.0
SRR3590749 PRJNA323467 Saccharomyces cerevisiae BY4743 Ribo-Seq 0.0
SRR3590750 PRJNA323467 Saccharomyces cerevisiae BY4743 Ribo-Seq 0.0
SRR3590751 PRJNA323467 Saccharomyces cerevisiae BY4743 Ribo-Seq 0.0
SRR3590752 PRJNA323467 Saccharomyces cerevisiae BY4743 Ribo-Seq 0.0
SRR3590753 PRJNA323467 Saccharomyces cerevisiae BY4743 Ribo-Seq 0.0
SRR3590770 PRJNA323467 Saccharomyces cerevisiae 0.0 Ribo-Seq 0.0
SRR3590771 PRJNA323467 Saccharomyces cerevisiae 0.0 Ribo-Seq 0.0
SRR3590772 PRJNA323467 Saccharomyces cerevisiae 0.0 Ribo-Seq 0.0
SRR3590773 PRJNA323467 Saccharomyces cerevisiae 0.0 Ribo-Seq 0.0
SRR3590774 PRJNA323467 Saccharomyces cerevisiae 0.0 Ribo-Seq 0.0
SRR3590775 PRJNA323467 Saccharomyces cerevisiae 0.0 Ribo-Seq 0.0
SRR3590776 PRJNA323467 Saccharomyces cerevisiae 0.0 Ribo-Seq 0.0
SRR3590777 PRJNA323467 Saccharomyces cerevisiae 0.0 Ribo-Seq 0.0
SRR3590778 PRJNA323467 Saccharomyces cerevisiae 0.0 Ribo-Seq 0.0
SRR3590779 PRJNA323467 Saccharomyces cerevisiae 0.0 Ribo-Seq 0.0
SRR3590780 PRJNA323467 Saccharomyces cerevisiae 0.0 Ribo-Seq 0.0
SRR3590781 PRJNA323467 Saccharomyces cerevisiae 0.0 Ribo-Seq 0.0
SRR3590782 PRJNA323467 Saccharomyces cerevisiae 0.0 Ribo-Seq 0.0
SRR3590783 PRJNA323467 Saccharomyces cerevisiae 0.0 Ribo-Seq 0.0
SRR3590784 PRJNA323467 Saccharomyces cerevisiae 0.0 Ribo-Seq 0.0
SRR3590785 PRJNA323467 Saccharomyces cerevisiae 0.0 Ribo-Seq 0.0
Run Accession Study Accession Scientific Name Cell Line Library Type Treatment GWIPS-viz Trips-Viz Reads BAM BigWig (F) BigWig (R)

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