Mills et al. 2016 (PRJNA339577)
General Details
| Title | Ribosome profiling of human platelets, reticulocytes, and in vitro cell lines |
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| Organism | |
| Number of Samples | 32 |
| Release Date | 2016/08/19 00:00 |
| Sequencing Types | |
| Protocol Details |
Study Links
| GWIPS-viz | Trips-Viz |
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| Visit GWIPS-viz |
Repository Details
| SRA | SRP082436 |
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| ENA | SRP082436 |
| GEO | |
| BioProject | PRJNA339577 |
Publication
| Title | |
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| Authors | Mills EW,Wangen J,Green R,Ingolia NT |
| Journal | Cell reports |
| Publication Date | 2016 Sep 27 |
| Abstract | Protein synthesis continues in platelets and maturing reticulocytes, although these blood cells lack nuclei and do not make new mRNA or ribosomes. Here, we analyze translation in primary human cells from anucleate lineages by ribosome profiling and uncover a dramatic accumulation of post-termination unrecycled ribosomes in the 3' UTRs of mRNAs. We demonstrate that these ribosomes accumulate as a result of the natural loss of the ribosome recycling factor ABCE1 during terminal differentiation. Induction of the ribosome rescue factors PELO and HBS1L is required to support protein synthesis when ABCE1 levels fall and for hemoglobin production during blood cell development. Our observations suggest that this distinctive loss of ABCE1 in anucleate blood lineages could sensitize them to defects in ribosome homeostasis, perhaps explaining in part why genetic defects in the fundamental process of ribosome production ('ribosomopathies') often affect hematopoiesis specifically. Copyright © 2016 The Author(s). Published by Elsevier Inc. All rights reserved. |
| PMC | PMC5111367 |
| PMID | 27681415 |
| DOI |
| Run Accession | Study Accession | Scientific Name | Cell Line | Library Type | Treatment | GWIPS-viz | Trips-Viz | Reads | BAM | BigWig (F) | BigWig (R) | ||
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| SRR4045263 | PRJNA339577 | Homo sapiens | primary cell type | Ribo-Seq |  |
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| SRR4045264 | PRJNA339577 | Homo sapiens | primary cell type | Ribo-Seq | |
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| SRR4045265 | PRJNA339577 | Homo sapiens | primary cell type | Ribo-Seq | |
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| SRR4045266 | PRJNA339577 | Homo sapiens | primary cell type | Ribo-Seq | |
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| SRR4045267 | PRJNA339577 | Homo sapiens | primary cell type | Ribo-Seq | |
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| SRR4045268 | PRJNA339577 | Homo sapiens | primary cell type | Ribo-Seq | |
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| SRR4045269 | PRJNA339577 | Homo sapiens | primary cell type | Ribo-Seq | |
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| SRR4045270 | PRJNA339577 | Homo sapiens | K562 | |
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| SRR4045271 | PRJNA339577 | Homo sapiens | K562 | |
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| SRR4045272 | PRJNA339577 | Homo sapiens | K562 | |
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| SRR4045273 | PRJNA339577 | Homo sapiens | K562 | |
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| SRR4045274 | PRJNA339577 | Homo sapiens | K562 | |
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| SRR4045275 | PRJNA339577 | Homo sapiens | K562 | |
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| SRR4045276 | PRJNA339577 | Homo sapiens | cell line | Ribo-Seq | |
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| SRR4045277 | PRJNA339577 | Homo sapiens | cell line | Ribo-Seq | |
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| SRR4045278 | PRJNA339577 | Homo sapiens | cell line | Ribo-Seq | |
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| SRR4045279 | PRJNA339577 | Homo sapiens | K562 | |
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| SRR4045280 | PRJNA339577 | Homo sapiens | K562 | |
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| SRR4045281 | PRJNA339577 | Homo sapiens | K562 | |
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| SRR4045282 | PRJNA339577 | Homo sapiens | K562 | |
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| SRR4045283 | PRJNA339577 | Homo sapiens | K562 | |
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| SRR4045284 | PRJNA339577 | Homo sapiens | K562 | |
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| SRR4045285 | PRJNA339577 | Homo sapiens | K562 | |
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| SRR4045286 | PRJNA339577 | Homo sapiens | K562 | |
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| SRR4045287 | PRJNA339577 | Homo sapiens | K562 | |
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| SRR4045288 | PRJNA339577 | Homo sapiens | K562 | |
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| SRR4045289 | PRJNA339577 | Homo sapiens | K562 | |
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| SRR4045290 | PRJNA339577 | Homo sapiens | K562 | |
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| SRR4045291 | PRJNA339577 | Homo sapiens | cell line | |
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| SRR4045292 | PRJNA339577 | Homo sapiens | cell line | |
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| SRR4045293 | PRJNA339577 | Homo sapiens | cell line | |
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| SRR4045294 | PRJNA339577 | Homo sapiens | cell line | |
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| Run Accession | Study Accession | Scientific Name | Cell Line | Library Type | Treatment | GWIPS-viz | Trips-Viz | Reads | BAM | BigWig (F) | BigWig (R) |
ⓘ For more Information on the columns shown here see: About