Datasets

Title	Translation of poly(A) tails leads to precise mRNA cleavage and widespread ribosome rescue
Organism
Number of Samples	15
Release Date	2016/08/23 00:00
Sequencing Types
Protocol Details

Title
Authors	Guydosh NR, Green R
Journal	RNA (New York, N.Y.)
Publication Date	2017 May
Abstract	Translation of poly(A) tails leads to mRNA cleavage but the mechanism and global pervasiveness of this 'nonstop/no-go' decay process is not understood. Here we performed ribosome profiling (in a yeast strain lacking exosome function) of short 15-18 nucleotides mRNA footprints to identify ribosomes stalled at 3' ends of mRNA decay intermediates. In this background, we found mRNA cleavage extending hundreds of nucleotides upstream of ribosome stalling in poly(A) and predominantly in one reading frame. These observations suggest that decay-triggering endonucleolytic cleavage is closely associated with the ribosome. Surprisingly, ribosomes appeared to accumulate (i.e., stall) in the transcriptome when as few as three consecutive ORF-internal lysine codons were positioned in the A, P, and E sites though significant mRNA degradation was not observed. Endonucleolytic cleavage was found, however, at sites of premature polyadenylation (encoding polylysine) and rescue of the ribosomes stalled at these sites was dependent on Dom34. These results suggest this process may be critical when changes in the polyadenylation site occur during development, tumorigenesis, or when translation termination/recycling is impaired. © 2017 Guydosh and Green; Published by Cold Spring Harbor Laboratory Press for the RNA Society.
PMC	PMC5393183
PMID	28193672
DOI

Guydosh et al. 2017 (PRJNA339793)