Datasets

Title	YTHDF3 facilitates translation and decay of the N6-methyladenosine-modified RNA
Organism
Number of Samples	8
Release Date	2016/08/30 00:00
Sequencing Types
Protocol Details

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Title
Authors	Shi H,Wang X,Lu Z,Zhao BS,Ma H,Hsu PJ,Liu C,He C
Journal	Cell research
Publication Date	2017 Mar
Abstract	N 6 -methyladenosine (m 6 A) is the most abundant internal modification in eukaryotic messenger RNAs (mRNAs), and plays important roles in cell differentiation and tissue development. It regulates multiple steps throughout the RNA life cycle including RNA processing, translation, and decay, via the recognition by selective binding proteins. In the cytoplasm, m 6 A binding protein YTHDF1 facilitates translation of m 6 A-modified mRNAs, and YTHDF2 accelerates the decay of m 6 A-modified transcripts. The biological function of YTHDF3, another cytoplasmic m 6 A binder of the YTH (YT521-B homology) domain family, remains unknown. Here, we report that YTHDF3 promotes protein synthesis in synergy with YTHDF1, and affects methylated mRNA decay mediated through YTHDF2. Cells deficient in all three YTHDF proteins experience the most dramatic accumulation of m 6 A-modified transcripts. These results indicate that together with YTHDF1 and YTHDF2, YTHDF3 plays critical roles to accelerate metabolism of m 6 A-modified mRNAs in the cytoplasm. All three YTHDF proteins may act in an integrated and cooperative manner to impact fundamental biological processes related to m 6 A RNA methylation.
PMC	PMC5339834
PMID	28106072
DOI

Shi et al. 2016 (PRJNA340948)