Sun et al. 2017 (PRJNA361113)

General Details

Title Domestic chickens activate a piRNA defense against avian leukosis virus
Organism
Number of Samples 3
Release Date 2017/01/12 00:00
Sequencing Types
Protocol Details

Study Links

Repository Details

SRA SRP096694
ENA SRP096694
GEO
BioProject PRJNA361113

Publication

Title
Authors Sun YH,Xie LH,Zhuo X,Chen Q,Ghoneim D,Zhang B,Jagne J,Yang C,Li XZ
Journal eLife
Publication Date 2017 Apr 6
Abstract PIWI-interacting RNAs (piRNAs) protect the germ line by targeting transposable elements (TEs) through the base-pair complementarity. We do not know how piRNAs co-evolve with TEs in chickens. Here we reported that all active TEs in the chicken germ line are targeted by piRNAs, and as TEs lose their activity, the corresponding piRNAs erode away. We observed de novo piRNA birth as host responds to a recent retroviral invasion. Avian leukosis virus (ALV) has endogenized prior to chicken domestication, remains infectious, and threatens poultry industry. Domestic fowl produce piRNAs targeting ALV from one ALV provirus that was known to render its host ALV resistant. This proviral locus does not produce piRNAs in undomesticated wild chickens. Our findings uncover rapid piRNA evolution reflecting contemporary TE activity, identify a new piRNA acquisition modality by activating a pre-existing genomic locus, and extend piRNA defense roles to include the period when endogenous retroviruses are still infectious.
PMC PMC5383398
PMID 28384097
DOI
Run Accession Study Accession Scientific Name Cell Line Library Type Treatment GWIPS-viz Trips-Viz Reads BAM BigWig (F) BigWig (R)
SRR5172020 PRJNA361113 Gallus gallus Ribo-Seq
SRR5315024 PRJNA361113 Gallus gallus Ribo-Seq
SRR5315025 PRJNA361113 Gallus gallus Ribo-Seq
Run Accession Study Accession Scientific Name Cell Line Library Type Treatment GWIPS-viz Trips-Viz Reads BAM BigWig (F) BigWig (R)

ⓘ For more Information on the columns shown here see: About