Wang et al. 2020 (PRJNA375080)

General Details

Title A spatio-temporal translatome of mouse tissue development
Organism
Number of Samples 24
Release Date 2017/02/16 00:00
Sequencing Types
Protocol Details

Study Links

Repository Details

SRA SRP100063
ENA SRP100063
GEO GSE94982
BioProject PRJNA375080

Publication

Title
Authors Wang H,Wang Y,Yang J,Zhao Q,Tang N,Chen C,Li H,Cheng C,Xie M,Yang Y,Xie Z
Journal Nucleic acids research
Publication Date 2021 Jun 21
Abstract The current understanding of how overall principles of translational control govern the embryo-to-adult transition in mammals is still far from comprehensive. Herein we profiled the translatomes and transcriptomes of six tissues from the mice at embryonic and adult stages and presented the first report of tissue- and stage-specific translational landscape in mice. We quantified the extent of gene expression divergence among different expression layers, tissues and stages, detected significant changes in gene composition and function underlying these divergences and revealed the changing architecture of translational regulation. We further showed that dynamic translational regulation can be largely achieved via modulation of translational efficiency. Translational efficiency could be altered by alternative splicing (AS), upstream and downstream open reading frames (uORFs and dORFs). We revealed AS-mediated translational repression that was exerted in an event type-dependent manner. uORFs and dORFs exhibited mutually exclusive usage and the opposing effects of translational regulation. Furthermore, we discovered many novel microproteins encoded by long noncoding RNAs and demonstrated their regulatory potential and functional relevance. Our data and analyses will facilitate a better understanding of the complexity of translation and translational regulation across tissue and stage spectra and provide an important resource to the translatome research community. © The Author(s) 2021. Published by Oxford University Press on behalf of Nucleic Acids Research.
PMC PMC8216458
PMID 34107020
DOI
Run Accession Study Accession Scientific Name Cell Line Library Type Treatment GWIPS-viz Trips-Viz Reads BAM BigWig (F) BigWig (R)
SRR11218272 PRJNA375080 Mus musculus Ribo-Seq
SRR11218273 PRJNA375080 Mus musculus Ribo-Seq
SRR11218268 PRJNA375080 Mus musculus Ribo-Seq
SRR11218269 PRJNA375080 Mus musculus Ribo-Seq
SRR11218270 PRJNA375080 Mus musculus Ribo-Seq
SRR11218271 PRJNA375080 Mus musculus Ribo-Seq
SRR11218274 PRJNA375080 Mus musculus Ribo-Seq
SRR11218275 PRJNA375080 Mus musculus Ribo-Seq
SRR11218276 PRJNA375080 Mus musculus Ribo-Seq
SRR11218277 PRJNA375080 Mus musculus Ribo-Seq
SRR11218278 PRJNA375080 Mus musculus Ribo-Seq
SRR11218279 PRJNA375080 Mus musculus Ribo-Seq
SRR5262884 PRJNA375080 Mus musculus Ribo-Seq
SRR5262885 PRJNA375080 Mus musculus Ribo-Seq
SRR5262886 PRJNA375080 Mus musculus Ribo-Seq
SRR5262887 PRJNA375080 Mus musculus Ribo-Seq
SRR5262888 PRJNA375080 Mus musculus Ribo-Seq
SRR5262889 PRJNA375080 Mus musculus Ribo-Seq
SRR5262890 PRJNA375080 Mus musculus Ribo-Seq
SRR5262891 PRJNA375080 Mus musculus Ribo-Seq
SRR5262892 PRJNA375080 Mus musculus Ribo-Seq
SRR5262893 PRJNA375080 Mus musculus Ribo-Seq
SRR5262894 PRJNA375080 Mus musculus Ribo-Seq
SRR5262895 PRJNA375080 Mus musculus Ribo-Seq
Run Accession Study Accession Scientific Name Cell Line Library Type Treatment GWIPS-viz Trips-Viz Reads BAM BigWig (F) BigWig (R)

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