Lalanne et al. 2018 (PRJNA376419)

General Details

Title Evolutionary Convergence of Pathway-specific Enzyme Expression Stoichiometry
Organism
Number of Samples 5
Release Date 2017/02/22 00:00
Sequencing Types
Protocol Details

Study Links

Repository Details

SRA SRP100536
ENA SRP100536
GEO GSE95211
BioProject PRJNA376419

Publication

Title
Authors Lalanne JB,Taggart JC,Guo MS,Herzel L,Schieler A,Li GW
Journal Cell
Publication Date 2018 Apr 19
Abstract Coexpression of proteins in response to pathway-inducing signals is the founding paradigm of gene regulation. However, it remains unexplored whether the relative abundance of co-regulated proteins requires precise tuning. Here, we present large-scale analyses of protein stoichiometry and corresponding regulatory strategies for 21 pathways and 67-224 operons in divergent bacteria separated by 0.6-2 billion years. Using end-enriched RNA-sequencing (Rend-seq) with single-nucleotide resolution, we found that many bacterial gene clusters encoding conserved pathways have undergone massive divergence in transcript abundance and architectures via remodeling of internal promoters and terminators. Remarkably, these evolutionary changes are compensated post-transcriptionally to maintain preferred stoichiometry of protein synthesis rates. Even more strikingly, in eukaryotic budding yeast, functionally analogous proteins that arose independently from bacterial counterparts also evolved to convergent in-pathway expression. The broad requirement for exact protein stoichiometries despite regulatory divergence provides an unexpected principle for building biological pathways both in nature and for synthetic activities. Copyright © 2018 Elsevier Inc. All rights reserved.
PMC PMC5978003
PMID 29606352
DOI
Run Accession Study Accession Scientific Name Cell Line Library Type Treatment GWIPS-viz Trips-Viz Reads BAM BigWig (F) BigWig (R)
SRR5278491 PRJNA376419 Bacillus subtilis Ribo-Seq
SRR5278492 PRJNA376419 Bacillus subtilis Ribo-Seq
SRR5278493 PRJNA376419 Bacillus subtilis Ribo-Seq
SRR5278494 PRJNA376419 Vibrio natriegens Ribo-Seq
SRR6652803 PRJNA376419 Bacillus subtilis Ribo-Seq
Run Accession Study Accession Scientific Name Cell Line Library Type Treatment GWIPS-viz Trips-Viz Reads BAM BigWig (F) BigWig (R)

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