Datasets

Title	Global mRNA polarization regulates translation efficiency in the intestinal epithelium [Ribo-Seq]
Organism
Number of Samples	6
Release Date	2017/02/27 00:00
Sequencing Types
Protocol Details

Title
Authors	Moor AE,Golan M,Massasa EE,Lemze D,Weizman T,Shenhav R,Baydatch S,Mizrahi O,Winkler R,Golani O,Stern-Ginossar N,Itzkovitz S
Journal	Science (New York, N.Y.)
Publication Date	2017 Sep 22
Abstract	Asymmetric messenger RNA (mRNA) localization facilitates efficient translation in cells such as neurons and fibroblasts. However, the extent and importance of mRNA polarization in epithelial tissues are unclear. Here, we used single-molecule transcript imaging and subcellular transcriptomics to uncover global apical-basal intracellular polarization of mRNA in the mouse intestinal epithelium. The localization of mRNAs did not generally overlap protein localization. Instead, ribosomes were more abundant on the apical sides, and apical transcripts were consequently more efficiently translated. Refeeding of fasted mice elicited a basal-to-apical shift in polarization of mRNAs encoding ribosomal proteins, which was associated with a specific boost in their translation. This led to increased protein production, required for efficient nutrient absorption. These findings reveal a posttranscriptional regulatory mechanism involving dynamic polarization of mRNA and polarized translation. Copyright © 2017 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works.
PMC	PMC5955215
PMID	28798045
DOI

Moor et al. 2017 (PRJNA377088)