Slobodin et al. 2017 (PRJNA379292)

Title	RNA_seq and Ribo_seq analyses of control and CPT-treated MCF7 cells
Organism
Number of Samples	4
Release Date	2017/03/15 00:00
Sequencing Types
Protocol Details

GWIPS-viz	Trips-Viz

Title
Authors	Slobodin B,Han R,Calderone V,Vrielink JAFO,Loayza-Puch F,Elkon R,Agami R
Journal	Cell
Publication Date	2017 Apr 6
Abstract	Transcription and translation are two main pillars of gene expression. Due to the different timings, spots of action, and mechanisms of regulation, these processes are mainly regarded as distinct and generally uncoupled, despite serving a common purpose. Here, we sought for a possible connection between transcription and translation. Employing an unbiased screen of multiple human promoters, we identified a positive effect of TATA box on translation and a general coupling between mRNA expression and translational efficiency. Using a CRISPR-Cas9-mediated approach, genome-wide analyses, and in vitro experiments, we show that the rate of transcription regulates the efficiency of translation. Furthermore, we demonstrate that m 6 A modification of mRNAs is co-transcriptional and depends upon the dynamics of the transcribing RNAPII. Suboptimal transcription rates lead to elevated m 6 A content, which may result in reduced translation. This study uncovers a general and widespread link between transcription and translation that is governed by epigenetic modification of mRNAs. Copyright © 2017 The Authors. Published by Elsevier Inc. All rights reserved.
PMC	PMC5388891
PMID	28388414
DOI

ⓘ For more Information on the columns shown here see: About