Slobodin et al. 2017 (PRJNA379447)
General Details
| Title | RNA_seq and Ribo_seq analyses applied to PC9 and H1933 human cancer cell lines |
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| Organism | |
| Number of Samples | 2 |
| Release Date | 2017/03/16 00:00 |
| Sequencing Types | |
| Protocol Details |
Study Links
| GWIPS-viz | Trips-Viz |
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Repository Details
| SRA | SRP102021 |
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| ENA | SRP102021 |
| GEO | GSE96716 |
| BioProject | PRJNA379447 |
Publication
| Title | |
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| Authors | Slobodin B, Han R, Calderone V, Vrielink JAFO, Loayza-Puch F, Elkon R, Agami R |
| Journal | Cell |
| Publication Date | 2017 Apr 6 |
| Abstract | Transcription and translation are two main pillars of gene expression. Due to the different timings, spots of action, and mechanisms of regulation, these processes are mainly regarded as distinct and generally uncoupled, despite serving a common purpose. Here, we sought for a possible connection between transcription and translation. Employing an unbiased screen of multiple human promoters, we identified a positive effect of TATA box on translation and a general coupling between mRNA expression and translational efficiency. Using a CRISPR-Cas9-mediated approach, genome-wide analyses, and in vitro experiments, we show that the rate of transcription regulates the efficiency of translation. Furthermore, we demonstrate that m 6 A modification of mRNAs is co-transcriptional and depends upon the dynamics of the transcribing RNAPII. Suboptimal transcription rates lead to elevated m 6 A content, which may result in reduced translation. This study uncovers a general and widespread link between transcription and translation that is governed by epigenetic modification of mRNAs. Copyright © 2017 The Authors. Published by Elsevier Inc. All rights reserved. |
| PMC | PMC5388891 |
| PMID | 28388414 |
| DOI |
| Run Accession | Study Accession | Scientific Name | Cell Line | Library Type | Treatment | GWIPS-viz | Trips-Viz | Reads | BAM | BigWig (F) | BigWig (R) | ||
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| SRR5350744 | PRJNA379447 | Homo sapiens | PC9 | Ribo-Seq | Cycloheximide |  |
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| SRR5350745 | PRJNA379447 | Homo sapiens | H1933 | Ribo-Seq | Cycloheximide | |
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| Run Accession | Study Accession | Scientific Name | Cell Line | Library Type | Treatment | GWIPS-viz | Trips-Viz | Reads | BAM | BigWig (F) | BigWig (R) |
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